猪H-FABP基因多态片段的序列分析

Sequence Analysis of Polymorphic Fragments of Porcine H-FABP Gene

利用PCR RFLP方法在猪H FABP基因内确定了 3个变异酶切位点 ,分别为 5′ -上游区HinfI RFLP、内含子 2的HaeIII RFLP和HinfI RFLP(HinfI和HinfI 代表不同区域的HinfI酶切反应 )。对每个多态片段进行克隆测序分析 ,结果表明 ,5′ -上游区HinfI RFLP是由于 132 4位的碱基T→C的突变引起 ;在内含子 2中 ,HaeIII RFLP变异酶切位点在 1811位 ,发生了C到G的突变 ;HinfI RFLP是由于

Three variant restriction sites of porcine H-FABP gene,including HinfI-RFLP in 5′-upsream,HaeIII-RFLP and HinfI *-RFLP in intron 2,were confirmed by PCR-RFLP method.The polymorphic fragments were cloned and sequenced.The results revealed a single nucleotide substitution of T→C at position 1324 for HinfI-RFLP,C→G at position 1811 for HaeIII-RFLP and T→C at position 1970 for HinfI *-RFLP,respectively.