急性心肌梗死患者凝血酶原基因G20210A变异频度分析

FⅡG20210A GENE FREQUENCY IN CHINESE PEOPLEWITH ACUTE MYOCARDIAL INFARCTION

目的 :探讨FⅡG2 0 2 10A突变在中国人群中的分布频率及其与急性心肌梗塞之间的联系。方法 :运用FⅡG2 0 2 10A等位基因特异性引物PCR ,结合限制性内切酶技术及聚丙烯酰胺凝胶电泳等方法检测 97例急性心肌梗塞患者及 10 7例正常人对照的FⅡG2 0 2 10A基因频率。结果 :未检出 1例FⅡG2 0 2 10A基因突变者 ,提示被检病例及正常人FⅡG2 0 2 10A基因变异频率为 0。结论 :在中国人群中FⅡG2 0 2 10A基因变异罕见甚至缺如。FⅡG2 0 2 10A基因突变不足以成为中国人急性心肌梗塞的危险因素。

Objective:To investigate the frequency of prothrombin (FⅡ) gene G20210A variant and to assess whether this muation is related to acute myocardial infarction (AMI) in Chinese people.Methods:The G20210A mutant allele of the FⅡ gene was determined by PCR using allele specific primer,restriction enzyme HindⅢ digestion and polyacrylamide gel electrophoresis in 97 patients with AMI and 107 normal control subjects. Results:The patients and healthy controls were all homozygous for the normal FⅡG20210G allele,and there was no FⅡG20210A variant. Conclusions:FⅡ gene G20210A mutant allele is rare or absent in Chinese people,FⅡG20210A mutation was not significantly associated with AMI.