凝血酶原基因G20210 A多态性与心肌梗死和脑卒中的相关性

Association between FⅡG20210 A gene polymorphism and acute myocardial infarction and ischemic stroke

目的 :研究凝血酶原基因G2 0 2 10A(FⅡG2 0 2 10A)多态性在中国人群中的分布频率及其与急性心肌梗死和缺血性脑卒中之间的相关性。方法 :运用PCR、限制性内切酶消化及聚丙烯酰胺凝胶电泳等方法检测 173例缺血性脑卒中患者、97例急性心肌梗死患者及 10 7例正常人对照的FⅡG2 0 2 10A等位基因。结果 :未检出一例FⅡG2 0 2 10A基因突变者 ,提示被检病例及正常人FⅡG2 0 2 10A基因变异频率为 0。结论 :在中国人群中 ,FⅡG2 0 2 10A基因变异罕见甚至缺如。FⅡG2 0 2 10A基因多态性不足以成为中国人缺血性脑卒中与急性心肌梗死的危险因素。

Objective:To ascertain the frequency of prothrombin(FⅡ) gene G20210A variant and to investigate whether this polymorphism is related to arterial thrombosis in Chinese people.Method: PCR, restriction enzyme HindⅢ digestion and polyacrylamide gel electrophoresis were used to determined the prevalence of the G20210A mutation in 97 patients with acute myocardial infarction(AMI), 173 patients with ischemic stroke and normal control subjects.Result: the patients and healthy controls were all homozygous for the normal FⅡG20210G allele, and there was no FⅡG20210A variant.Conclusion: FⅡgene G20210A mutant allele is rare or absent in Chinese people, FⅡG20210A polymorphlism was not significantly associated with AIM and AIS.