针灸内关预处理对心肌缺血再灌注损伤兔血清NO、NOS及腺苷含量的影响

The Effect of Acupuncture at Neiguan(PC6) Pretreatment on the Expression of Blood Serum NO,NOS,and Adenosine in Rabbits with MIR

目的观察针灸内关预处理对心肌缺血再灌注损伤兔血清一氧化氮(NO)、一氧化氮合酶(NOS)及腺苷含量的影响。方法将32只新西兰大耳白兔随机分为假手术组、缺血再灌注组、电针组和艾灸组。采用结扎左冠状动脉前降支40 min再灌注60 min的方法,建立心肌缺血再灌注损伤模型。假手术组于末次捆绑结束48 h后开胸,穿线静置40 min,拔线,继续静置60 min后取材;缺血再灌注组于末次捆绑结束后48 min行缺血再灌注后取材,电针组和艾灸组分别在针刺、艾灸内关穴之后48 h再行缺血再灌注。高效液相色谱法测定血清腺苷含量,采用硝酸还原酶化学比色法检测血清NO、NOS的含量。结果缺血再灌注组血清NO、NOS及腺苷的含量较假手术组相比明显下降(P<0.05);电针组与艾灸组血清NO、NOS及腺苷的含量较缺血再灌注模型组相比均明显升高(P<0.01),而电针组与艾灸组两组间无明显差异(P>0.05)。结论电针、艾灸内关穴预处理可以提高兔血清中NO、NOS及腺苷的含量,即可以增强延迟性保护机制的细胞信号转导通路中触发物质NO、NOS、腺苷的活性和含量,表明了针灸预处理内关穴可以在48 h这一延迟时相产生针对缺血再灌注损伤心肌的保护作用。

Objective： To observe the effect of acupuncture at Neiguan （PC6） pretreatment on the expression of NO, NOS, and adenosine. Methods： 32 New Zealand big-ear rabbits were randomly divided into 4 groups： shamoperation group,ischemia reperfusion group, electric acupuncture preconditioning group and moxibustion pretreatment group. Myocardial ischemia-reperfusion injury model was established by the method of Ligating the anterior descending branch of the left coronary artery for 40 min and then reperfusing for 60 min. After the last bunding for 48 h, rabbits in sham-operation group were chest-opened, threaded and standed for 40 min,then the line was pulled out and standed for 60 minutes,then samples were selected. After the last bunding for 48 mins,rabbits in ischemia reperfusion group were conducted ischemia-reperfusion,then samples were selected ;rabbits in electric acupuncture preconditioning and moxibustion pretreatment group were acupunctured at Neiguan （PC6） acupoint respectively. After that for 48 h,ischemia-reperfusion was conducted. Serum adenosine content was determined by high performance liquid chromatography;the content of serum NO and NOS was determined by ELISA method. Results： NO,NOS,and adenosine contents of ischemia-reperfusion group decreased significantly than those of control sham-operation group with statistical difference （P 〈 0.05）;NO, NOS,and adenosine contents of electric acupuncture preconditioning and moxibustion pretreatment group increased significantly than those of control ischemia-reperfusion group with obviously statistical difference （P〈 0.01 ）, but there was no significantly difference between electric acupuncture preconditioning and moxibustion pretreatment group （P〉 0.05）. Conclusion： Electric acupuncture pretreatment and moxibustion pretreatment can increase the release of endogenous protection materials in serum of MIRI rabbits such as NO ,NOS ,and adenosine ,which indicates the protective effect of acupuncture at Neiguan（PC6） pretreatment on ischemical reperfusion injury in 48 h.