毛细管电泳-电感耦合等离子体质谱(CE-ICP-MS)联用测定干海产品中的六种砷形态化合物

Analysis of arsenic species in dry seafood products by capillary electrophoresis-inductively coupled plasma mass spectrometry

采用电感耦合等离子体质谱（ICP—MS）测定了鱿鱼、银鱼、沙丁鱼、虾、牡蛎、蛤蜊、紫菜、羊栖菜、海带、浒苔10种干海产品中的砷总量，建立了毛细管电泳（CE）与ICP—MS联用测定亚砷酸盐As（III）、砷酸盐As（V）、甲基砷酸（MMA）、二甲基砷酸（DMA）、砷甜菜碱（AsB）和砷胆碱（AsC）六种砷形态化合物的分析方法。结果发现除了浒苔其余干海产品中砷总量均超出了无机砷允许限值（GB2762—2005），形态分析结果表明干海产品中主要是有机砷，萃取液中无机砷含量仅占砷总量的0—15.2％。该方法简单方便、快速，样品消耗量少，而且具有较好的准确性，适用于其他生物样品中砷形态化合物的分析。

The total concentrations of arsenic in ten kinds of dry seafood products （DSPs）were determined by inductively coupled plasma mass spectrometry （ ICP- MS）, including Squid, Whitebait, Sardine, Shrimp, Oyster, Clam,Laver, Sargassum fusiform, Kelp, Enteromorpha prolifera. A method using capillary electrophoresis （CE） hyphenated with ICP-MS was established for the detection of arsenobetaine（AsB）, arsenocholine（AsC）, arsenite （ As （III） ）, arsenate（ As（V） ）, monomethylarsonic acid （MMA） and dimethylarsinic acid （DMA） .The results showed that total concentrations of arsenic in these DSPs were found to be higher than the maximum levels of contaminants in foods（GB2762-2005）except enteromorpha prolifera.For the species, organic arsenic was the main species in these DSPs,while the inorganic arsenic in the extraction solutions were very low and took approximate 0 to 15.2% of total arsenic.With the advantages of simple operation, less time and samples and accurate result,the method can be used to determine other biological samples.