体外受精优质囊胚培养与D_2D_3胚胎卵裂球数及碎片评级的相关性

Correlation between IVF blastocysts quality cultivation and D_2D_3 embryo cleavage count and debris rating

目的：观察体外受精（IVF）治疗周期中体外培养优质囊胚形成情况，并分析其与D2D3胚胎卵裂球数及碎片评级关系。方法选择本院2013年11月-2014年1月卵裂期第3天（D3）冷冻后剩余胚胎，将其体外培养至囊胚期。观察优质囊胚形成情况。同时探讨其D2D3胚胎卵裂球数及碎片评级。结果 D3≥10个细胞的优质囊胚形成率为38．9％，7～9个细胞43．1％，比较无显著差异（P＞0．05）；7～9个细胞的优质囊胚形成率为43．1％，显著高于4～6个细胞10．2％，比较有显著差异（P＜0．05）；D24个细胞的优质囊胚形成率为43．6％，显著高于5个细胞28．9％，比较有显著差异（P＜0．05），并显著高于2～3个细胞数12．3％，比较有显著差异（P＜0．05）；5个细胞的优质囊胚形成率为28．9％，6个细胞23．4％，比较无显著差异（P＞0．05）；Ⅰ、Ⅱ级D3胚胎碎片的优质囊胚形成率为36．4％，显著高于Ⅲ、Ⅳ级6．1％，比较有显著差异（P＜0．05），Ⅰ、Ⅱ级D2胚胎碎片的优质囊胚形成率为35．3％，显著高于Ⅲ、Ⅳ级4．7％，比较有显著差异（P＜0．05）。结论延长体外受精培养可有效筛选发育潜力的胚胎。D3胚胎卵裂球数越多，优质囊胚越多。D2胚胎4个细胞，囊胚发育能力较好。Ⅰ、Ⅱ级D2、D3胚胎碎片的优质囊胚形成率较高。优质囊胚形成与D2、D3胚胎卵裂球数及碎片评级密切相关。

Obj ective To observe the position fertilization （IVF）treatment cycle of in vitro cultivation quality blastocyst formation and analyze its relationship with pieces of D2D3 embryo cleav-age count and rating.Methods Cleavage stage 3 day （D3 ）frozen surplus embryos were selected and cultured to blastocyst stage.Quality of blastocyst formation was observed,and at the same time their D2D3 embryo cleavage count and debris were discussed.Results The high quality D3 10 or more cells blastocyst formation rate was 38.9%,which was almost equal to 43.1%of 7 to 9 cells blastocyst formation rate （P〉0 .05 ）.7 to 9 cell quality blastocyst formation rate was 43 .1%, which was significantly higher than 10.2%of 4 to 6 cells （P〈0.05）.D2 4 cell quality blastocyst formation rate was 43.1%,which was significantly higher than 28.9%of 5 cells and 12.3%of 2 to 3 cells （P〈0 .05 ）.5 cells quality blastocyst formation rate was 28 .9%,which was almost equal to 23 .4%of 6 cells blastocyst formation rate （P〉0 .05 ）.Ⅰand classⅡD3 embryos pieces of high quality blastocyst formation rate was 36.4%,which was significantly higher than 6.1%of gradeⅢorⅣ（P〈0 .05 ）.Ⅰand class ⅡD2 embryos pieces of high quality blastocyst formation rate was 35.3%,which was significantly higher than 4.7% of grade Ⅲ or Ⅳ （P〈0.05）.Conclusion Extending IVF can effectively filter the embryo developmental potential.The more D3 embryo cleavage count ,the more high-quality blastocysts .D2 4 cells ,embryo blastocyst development ability is good.Ⅰand classⅡD2 and D3 embryos pieces of high quality blastocyst formation rate is higher.High quality blastocyst formation and D2 and D3 embryo cleavage count and debris are closely related to the ratings.