摘要
As members of a super gene family, WRKY transcription factors are widely distributed in higher plants. ln this study, bioinformatic analysis of WRKY35, a member of the WRKY gene family, was carried out. Results indicated that tran-scription factor WRKY35 harbors a WRKYGQK core domain and a Cys2His2 or Cys2His/Cys zinc finger in the 5’ end without transmembrane domain. After PCR amplification and restriction digestion, WRKY35 gene fragment was ligated to prokaryotic expression vector PET28. This study provided basis for expression anal-ysis of WRKY35 protein and subsequent functional identification of WRKY35 gene.
WRKY转录因子是一超级基因家族,数目众多,广泛存在于高等植物中。该研究通过对WRKY基因家族的一员WRKY35进行生物信息学分析,结果发现,在其5-存在一个WRKYGQK核心结构域,一个Cys2His2或Cys2His/Cys锌指型结构,无跨膜结构域;同时通过PCR扩增、酶切、连接等将WRKY35基因片段连接到原核表达载体PET28上,这可为WRKY35基因的蛋白质表达及其后续基因功能鉴定奠定基础。
基金
Supported by National Natural Science Foundation of China "Functional analysis of transcription factor AtWRKY28 in development and morphogenesis of Orychophragmus violaceus"(31360262)
Key Agricultural Science and Technology Project of Department of Science and Technology of Guizhou Province "Molecular Marker Development and Assisted Breeding of Recessive Epistatic Genic Male Sterile Lines of Rapeseed"(QKHNYZ[2012]3033)
Special Fund of Guizhou Academy of Agricultural Sciences "BnaC.Tic40 (tic40) and BnRf (rf) Marker-assisted Breeding of Recessive Genic Male Sterile Three Lines of Rapeseed"(QNKYYZX[2012]002)~~
