鉴定乳制品中厌氧芽胞杆菌的3种方法比较

Identification of anaerobic sporeforming Bacillus in dairy product by three methods

目的鉴定乳制品中的革兰氏阳性厌氧芽胞杆菌。方法采用生化鉴定、基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)鉴定和16S r DNA测序三种方法,对乳制品中5株革兰氏阳性厌氧芽胞杆菌进行鉴定。结果生化方法鉴定出3株菌,而飞行时间质谱鉴定和16S r DNA测序法对5株菌都给出了鉴定结果。3种方法仅对其中1株菌的鉴定结果一致;飞行时间质谱鉴定和16S r DNA测序的鉴定结果一致性程度高,共有4株菌的鉴定结果一致;而生化方法的鉴定结果与这两种方法差异较大。结论飞行时间质谱鉴定和16S r DNA测序可作为生化方法的补充,用于革兰氏阳性厌氧芽胞杆菌的快速鉴定。

Objective To identify the anaerobic sporeforming Bacillus in dairy products. Methods Five strains of gram-positive anaerobic sporeforming Bacilli in dairy product were identified by three methods,including Biochemical identification,MALDI-TOF MS and 16 S r DNA sequencing techniques. Results The biochemical method could identify three strains,whereas MALDI-TOF MS and 16 S r DNA sequencing techniques could identify all five Bacilli. The three methods gave the same identification results on only one of the five Bacilli. By contrast,MALDI-TOF MS and 16 S r DNA methods gave the same identification results on four of the five Bacilli,indicating that the two methods are highly identical in identifying the Bacillus. However,biochemical method had big variation in comparison with the other two methods.Conclusion MALDI-TOF MS and 16 S r DNA sequencing methods can be used as supplemental methods to the biochemical method in rapid identification of anaerobic sporeforming Bacillus.