摘要
Central Composite Design(CCD) and response surface methodology were used in the experiment to examine the combined effect of temperature(16-28℃) and salinity(18-42) on Hsp70 and IgM genes expression levels in turbot(Scophthalmus maximus) liver and kidney.The results showed that the coefficients of determination(R^2=0.965 2 for liver Hsp70,0.972 9 for kidney Hsp70,0.921 for liver IgM and 0.962 1 for kidney IgM) and probability values(P<0.01) were significant for the regression model.The interactive effect between temperature and salinity on liver Hsp70,kidney Hsp70 and liver IgM were not significant(P>0.05),while the interactive effect between temperature and salinity on kidney IgM was significant(P<0.01).The model equation could be used in practice for forecasting Hsp70 and IgM genes expression levels in the liver and kidney of juvenile turbot via applying statistical optimization of the response of interest,at which the maximum liver Hsp70,kidney Hsp70,liver IgM and kidney IgM of1.48,1.49,2.48,and 1.38,respectively,were reached.The present model may be valuable in assessing the feasibility of turbot farming at different geographic locations and,furthermore,could be a useful reference for scientists studying the immunity of turbot.
Central Composite Design （CCD） and response surface methodology were used in the experiment to examine the combined effect of temperature （16-28℃） and salinity （18-42） on Hsp70 and IgM genes expression levels in turbot （Scophthalmus maximus） liver and kidney. The results showed that the coefficients of determination （R^2=0.965 2 for liver Hsp70, 0.972 9 for kidney Hsp70, 0.921 for liver IgM and 0.962 1 for kidney IgM） and probability values （P〈0.01） were significant for the regression model. The interactive effect between temperature and salinity on liver Hsp70, kidney Hsp70 and liver IgM were not significant （P〉0.05）, while the interactive effect between temperature and salinity on kidney IgM was significant （P〈0.01）. The model equation could be used in practice for forecasting Hsp70 and IgM genes expression levels in the liver and kidney of juvenile turbot via applying statistical optimization of the response of interest, at which the maximum liver Hsp70, kidney Hsp70, liver IgM and kidney IgM of 1.48, 1.49, 2.48, and 1.38, respectively, were reached. The present model may be valuable in assessing the feasibility of turbot farming at different geographic locations and, furthermore, could be a useful reference for scientists studying the immunity of turbot.
基金
Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A408-8)
the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-50-G01)
