三七总皂苷配伍雷公藤多苷对胶原诱导关节炎大鼠心血管损伤的保护作用

Protective Effect of Panax Notoginsenosidum Combined with Tripterygium Glycosides on the Cardiovascular Injury in Rats with Collagen Induced Arthritis

目的观察植物药有效成分三七总皂苷(PNS)与雷公藤多苷(TG)配伍对胶原诱导关节炎(CIA)大鼠血管和心肌损伤的保护效应。方法 Wistar大鼠50只中选6只为对照组,其余皮内注射鸡II型胶原建立CIA模型,成模后随机分为模型组、雷公藤多苷组(TG)、TG+PNS(三七总皂苷)组,每组6只。对照组、模型组生理盐水灌胃,TG组雷公藤多苷灌胃,TG+PNS组三七总皂苷配伍雷公藤多苷灌胃,共42 d。治疗结束后,腹主动脉取血,采用蛋白芯片技术检测白介素(IL)-6、纤溶酶原激活物抑制剂(PAI-l)含量;采血后立即开胸,取胸主动脉及心脏,进行病理学观察。结果模型组及TG组、TG+PNS组造模第21天、给药4、6周后体重及体重增加值明显低于对照组(P<0.01),PAI-1、IL-6表达明显高于对照组(P<0.01);给药第4、6周TG+PNS组体重增加值明显高于模型组(P<0.05,P<0.01),血清PAI-1、IL-6水平显著降低(P<0.01),且PNS+TG组优于TG组(P<0.05)。模型组胸主动脉内皮不完整,可见炎细胞浸润、脂质沉积及泡沫细胞形成,心肌可见广泛炎性病变;TG组可见部分单核细胞、淋巴细胞等聚集,部分心肌细胞肥大;PNS+TG组内皮完整,未见明显病理改变,心肌仅见零星炎性细胞。结论 CIA大鼠存在心血管损伤,TG联合PNS在治疗关节炎的同时可能具有心血管保护作用。

Objective To observe the protective effect of Panax Notoginsenosidum（ PNS） combined with Tripterygium Glycosides（ TG） on the blood vessel and myocardial damage in rats with collagen induced arthritis（ CIA）. Methods Six rats from 50 Wistar rats were selected as control group,and the other rats were used to establish CIA models by giving intradermal injections of Chicken Collagen type II and successful models were randomly divided into model group（ n = 6）,TG group（ n =6） and TG ＋ PNS group（ n =6）. The control and model groups were lavaged with physiological saline,and TG group was lavaged with TG,while TG ＋ PNS group was lavaged with TG ＋ PNS for 42 d. After the treatment,the serum from abdominal aortas was obtained,and the contents of TNF-α（ tumor necrosis factor）,IL-6（ interleukin） and PAI-1（ plasminogen activator inhibitor） were detected using protein chip technology; thoracic cavity was opened immediately to get the thoracic aorta and cardiac tissue for pathological observation. Results On the 21 std after models establishment,and in the 4thand 6thweeks after administration,the increased values of body weight in model,TG and TG ＋ PNS groups were significantly lower than that in control group（ P〈0. 01）,while levels of PAI-1 and IL-6 were significantly higher to those in control group（ P〈0. 01）; in the 4^th and 6^th weeks after administration,the increased values of body weight in TG ＋ PNS group were significantly higher than those in model group（ P〈0. 05,P〈0. 01）,while levels of PAI-1 and IL-6 were significantly decreased（ P〈0. 01）,and the changes in PNS ＋ TG group were superior to those in TG group（ P〈0. 05）. The endothelium of thoracic aorta in model group was not integrated,and it had inflammatory cellular infiltration,lipidoses and foam cell formation with wide myocardial inflammatory lesions. Part of mononuclear and lymphocyte cell aggregation was found in TG group with part of myocardial cells hypertrophy. The endothelium of thoracic aorta in PNS ＋ TG group was integrated without pathological change,and sporadically inflammatory cells were found. Conclusion Rats with CIA suffer cardiovascular injury,and PNS ＋ TG treatment may have a protective effect on the cardiovascular injury in rats with CIA during the treatment of arthritis.