类球红细菌发酵生产类胡萝卜素条件优化

Optimization of fermentation conditions for carotenoids production by Rhodobacter sphaeroides

本文对类球红细菌3757产类胡萝卜素进行了发酵条件优化,结果得到了较优的培养基组成:葡萄糖2%,苹果酸钠0.5%,酵母浸粉1.3%,硫酸铵0.9%,磷酸氢二钾0.09%,磷酸二氢钾0.06%,生长因子溶液1%,p H 8.0;其中,生长因子溶液配方:维生素B1 0.1%,烟酰胺(VPP)0.1%,生物素0.0016%。较优培养条件为:接种量5%,转速200 r/min,种龄24 h,发酵温度32℃,发酵时间40 h。优化后类胡萝卜素产率较优化前提高了76.2%。

Fermentation medium and cultural conditions were optimized for the production of carotenoids by Rhodobacter sphaeroides 3757. The results showed that the optimized fermentation medium contained （ % ） ： glucose 2.0, sodium malate 0.5, yeast extract 1.3, ammonium sulfate 0.9, K2HPO4 0.09, KH2PO4 0.06, vitamin solution 1% and pH 8.0. The vitamin solution contained （ % ） ： VB1 0.1, nicotinamide （VPP） 0.1, and biotin 0. 0016. After inoculated with 5% of a 24-h-old inoculum culture and incubated at 32 ℃ for 40 h at 200 r/min, the yield of carotenoids was increased by 76.2% in contrast with that of its initial fermentation conditions.