摘要
A rapid and validated UPLC-MS method was developed for investigating the absorbed components of Paederia scandens(Lour.) Merrill(P.scandens) in rat plasma.The bioactive constituents in plasma samples from rats administrated orally with P.scandens extract were analyzed by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).Four prototype compounds were identified in rat serum as potential bioactive components of P.scandens by comparing their retention times and mass spectrometry data or by mass spectrometry analysis and retrieving the reference literatures.Glucuronidation after deglycosylation was the major metabolic pathway for the iridoid glycosides in P.scandens.These results showed that the methods had high sensitivity and resolution and were suitable for identifying the bioactive constituents in plasma after oral administration of P.scandens.providing helpful chemical information for further pharmacological and mechanistic researched on the P.scandens.
A rapid and validated UPLC-MS method was developed for investigating the absorbed components of Paederia scandens （Lour.） Merrill （P. scandens） in rat plasma. The bioactive constituents in plasma samples from rats administrated orally with P. scandens extract were analyzed by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS）. Four prototype compounds were identified in rat serum as potential bioactive components of P. scandens by comparing their retention times and mass spectrometry data or by mass spectrometry analysis and retrieving the reference literatures. Glucuronidation after deglycosylation was the major metabolic pathway for the iridoid glycosides in P. scandens. These results showed that the methods had high sensitivity and resolution and were suitable for identifying the bioactive constituents in plasma after oral administration of P. scandens, providing helpful chemical information for further pharmacological and mechanistic researched on the P. scandens.
基金
supported by the National Natural Science Foundation(Nos.81274013 and 81473315)
the National Natural foundationof the major program of China(No.81130069)
the Chinese National S&T Special Project on Major New Drug Innovation(No.2011ZX09307-002-01)
the program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT1140)
