秦皮薄层鉴别方法的改进

Improvement of TLC Identification Method for Fraxini Cortex

目的:对秦皮薄层鉴别的展开系统进行改进。方法:改进了原标准的展开系统,采用硅胶G板,以二氯甲烷-甲醇-甲酸(7∶0.5∶0.5)为展开剂,置紫外光灯(365 nm)下检视秦皮甲素、秦皮乙素、秦皮素。结果:在供试品色谱中,秦皮甲素、秦皮乙素、秦皮素斑点清晰,分离度好,重现性好。结论:该方法的展开系统减少了对环境的污染和对人体的伤害,可替代原标准用于秦皮的鉴别。

Objective： To improve the TLC identification method of Fraxini Cortex Methods： TLC developing system of the original quality standard was improved. Using silica gel G thin layer plate, aesculin, aesculetin and fraxetin were developed with dichloromethane-methanol-formic acid （ 7： 0. 5 ： 0. 5 ）, and detected under the uv lamp （ 365 nm ）. Results： In the charomatogram, the spots of aesculin, aesculetin and fraxetin were clear. The method was repeatable. Conclusion： The method is simple and environment friendly, could replace the original standard to identify Fraxini Cortex.