摘要
采用紫外线-Li Cl、硫酸二乙酯(DES)复合诱变,经平板菌落筛选、摇瓶筛选以及连续传代稳定性考察,从土曲霉(Aspergillus terreus)2433出发菌株获得一株衣康酸高产突变株土曲霉At DES-11,其产酸量达到48.22 g/L,较出发菌株提高了156.08%;糖酸转化率为61.82%,较出发菌株提高了101.36%,且连续传代5次后,遗传性状仍较稳定,产酸量稳定在48 g/L左右,菌落及菌丝形态基本不变。
By the compound mutation of UV-LiC1 and diethyl sulfate （DES）, fiat screening of colony, shaker screening, and stability test of continuous passage, a mutant strain of Aspergillus terreus At DES-11 with high yield of itaconic acid was obtained from A. terreus2433. Its itaconie acid productivity and conversion rate of glucose were 48.22 g/L and 61.82% with an increase of 156.08% and 101.36% compared with those of original strain ,respectively. And the heredity characteristics of the mutant strain were stable after cuhivation for five continuous generations.
出处
《生物质化学工程》
CAS
北大核心
2015年第3期13-16,共4页
Biomass Chemical Engineering
基金
中国林科院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2014QA023)
"十二五"国家科技支撑计划资助(2014BAD02B02)
关键词
土曲霉
衣康酸
复合诱变
Aspergillus terreus
itaconicacid
compound mutation
