超支化聚缩水甘油-羟喜树碱药物递送系统对人腺样囊性癌的体外抑制作用

Inhibition Effects of Hyperbranched Polyglycidol-HA Drug Delivery System on Human Adenoid Cystic Carcinoma in Vitro

目的:研究超支化聚缩水甘油-羟喜树碱药物递送系统(biodegradable hyperbranched polyglycerol-hydroxy camptothecin,d HPG-HA)对体外培养的人腺样囊性癌(高肺转移)细胞(salivary adenoid cystic carcinoma-lung metastases,SACC-LM)的抑制作用。方法 :细胞计数试剂盒(cell counting kit,CCK8)检测药物浓度为0.001、0.01、0.1、1、10、100μg/m L的羟喜树碱(hydroxy camptothecin,HA)和含等量HA的d HPG-HA,在24、48 h对肿瘤细胞的抑制作用;流式细胞仪检测浓度为0.001、0.01、0.1、1、10μg/m L的d HPG-HA是否能诱导SACC-LM细胞凋亡。结果:HA和d HPG-HA对SACC-LM细胞增殖均有明显抑制作用,且作用呈剂量依赖性和时间依赖性;d HPG-HA对SACC-LM细胞的细胞毒性明显低于HA;d HPG-HA能诱导SACC-LM细胞凋亡。结论:dHPG的构建能达到使药物HA缓释的作用并降低其细胞毒性。

Objective： To investigate the inhibition effects of hyperbranched polyglycidol-hydroxy camptothecin （dHPG-HA） drug delivery system on cultured human adenoid cystic carcinoma （high lung metastasis） cells （SACC-LM）. Methods：SACC-LM cells cultured in vitro were treated with different concentrations of hydroxy camptothecin （HA） and （dHPG-HA） which contained equal amounts of HA, and the proliferation inhibition of SACC-LM cells was detected with cell counting kit assay （CCK8）. Apoptosis of SACC-LM cells treated by various concentrations of HA and dHPG-HA was ascertained by flow cytometry. Results：The proliferation of SACC-LM cells was significantly reduced. And the effect was dose-dependent and time-dependent. In addition, the cytotoxicity of dHPG-HA on SACC-LM cells was obviously lower than free HA. Fur-thermore, dHPG-HA could induce apoptosis of SACC-LM cells effectively. In a word, the construction of drug carrier （dH-PG） on HA provided sustained release of drugs and reduced its cytotoxicity.