不同浓度DⅢ4调节绒毛外滋养细胞的生物学功能

Effect of DⅡ4 at different concentrations on the biological functions of extravillous trophoblasts

背景:早孕时期绒毛外滋养细胞功能的正常发挥对妊娠有着至关重要的影响,绒毛外滋养细胞功能异常时将导致子痫前期、胎儿生长受限及胎盘植入等多种妊娠期疾病。目的:探讨Notch信号家族配体Dll4对绒毛外滋养细胞的生物学功能的影响。方法:以不同质量浓度外源性Dll4(0,0.125,0.25,0.5,1.0 mg/L)处理人绒毛外滋养细胞系HTR-8/SVneo,以CCK-8试验检测细胞活性,Transwell实验检测细胞迁移、侵袭能力的改变。结果与结论:Dll4可明显促进绒毛外滋养细胞迁移与侵袭能力,而对细胞活性不产生显著影响;Dlll4对绒毛外滋养细胞侵袭功能的影响具有浓度依赖性。结果提示Dll4可通过调节绒毛外滋养细胞生物学功能,在妊娠过程很中发挥重要作用。

BACKGROUND：Extravil ous trophoblasts exert a crucial effect on early pregnancy. When extravil ous trophoblasts function abnormal y, many pregnancy diseases can develop, such as preeclampsia, fetal growth restriction and placental implantation. OBJECTIVE：To investigate the effect of Dl 4, a ligand of Notch signaling family, on biological functions of extravil ous trophoblasts. METHODS：Human extravil ous trophoblast cel lines, HTR-8/SVneo, were cultured with human recombined protein Dl 4 of different concentrations （0, 0.125, 0.25, 0.5, 1.0 mg/L）. Cel viability was tested by cel counting kit-8 assay. Transwel assay was applied to examine the changes in cel migration and invasion ability. RESULTS AND CONCLUSION：Dl 4 could significantly improve migration and invasion abilities of extravil ous trophoblasts, but had no effect on cel viability. In addition, Dl 4 regulated the invasion ability of extravil ous trophoblast in a dose-dependent manner. Above al , Dl 4 can play an important role in normal pregnancy by regulating the biological functions of extravil ous trophoblasts.