裸鼠颅内小细胞肺癌移植瘤模型的建立

Establish of intracranial small cell lung cancer xenograft model in nude mice

目的建立裸鼠颅内小细胞肺癌（SCLC）移植瘤模型,为今后开展SCLC相关实验研究提供动物模型。方法培养LTEP/P人SCLC细胞株,传代3~4代,收集细胞,并制成1×108/ml的细胞悬液共1 ml。14只裸鼠麻醉后,用牙科钻在裸鼠颅脑钻一小孔,然后用10μl微量注射器抽取3μl肿瘤细胞悬液,利用立体定位仪缓慢接种于BALB/C SPF裸鼠颅内,放置入SPF环境内继续饲养,观察7 d后脱颈处死,取完整脑组织,行病理检测。结果所有裸鼠在术后1 h内逐渐苏醒并恢复活动和饮食,第5天出现活动、饮食减少,第7天出现神软、萎靡、反应迟钝等症状,并死亡1只。病理检测证实所有裸鼠脑组织内可见SCLC细胞。结论通过立体定位仪向裸鼠颅内接种SCLC细胞能建立稳定可靠的裸鼠颅内SCLC移植瘤模型。

Objective To establish an intracranial xenograft model of small-cell lung cancer（SCLC） in nude mice for providing animal model for SCLC related experimental research. Methods LTEP/P human SCLC cells were cultured and passaged for 3~4 generations, and then 1ml cells of 1 ×108cells /ml were collected. After fourteen nude mice were anesthetized, a dental drill was used to drill a small hole in the skull of every mouse. And then, 3μl cells were extracted through a 10 μl syringe and slowly inoculated into the brain of BALB/C SPF mice by stereotaxic instrument. The mice were raised in SPF environment and killed by cervical dislocation at 7thday and brains tissues were taken for pathology detecting.Results All mice regained consciousness, gradually recovered and resumed eating within one hour after surgery. At 5th day, the mice appeared poor appetite and low active while the mice appeared sluggish, unresponsive and one mouse was died at 7thday. Pathological examination confirmed that SCLC cells were visible in the brain tissues of 13 nude mice. Conclusion It is feasible to establish a stable and reliable intracranial SCLC xenograft model by inoculating cells into the brains of nude mice through stereotaxic instrument.