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PIN2蛋白在水稻根尖细胞中定位方法的优化

Optimization of Localization Methods for PIN2 Protein in Cells of Root Tips of Rice
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摘要 PIN2蛋白作为生长素外排蛋白,在植物根的向地性生长中起重要作用。免疫定位法是一种可以将蛋白在植物细胞中快速准确定位的方法。本研究利用改良的免疫荧光定位法,对PIN2蛋白在水稻根尖细胞中的定位进行了观察,并与前人的两种方法进行比较。结果表明,与已有的方案比较,改良免疫荧光定位法可使PIN2蛋白在水稻根尖细胞中的位置快速准确地被观察到,且简化了操作步骤,操作方便快速,可广泛应用于植物其他蛋白的定位研究。 PIN2 as an auxin efflux protein plays important roles in geotropic growth of plant roots. Immunolocalization technique is a method which allows rapid and reliable in situ localization of proteins in plant cells. In this paper,the improved immunolocalization technique was used to localize the PIN2 in root tip cells of rice,and its effect was compared to the two existing methods. The results showed that the location of PIN2 could be more quickly and accurately determined in root tip cells of rice by the improved method; and the improved method simplified the operation procedures and was more convenient,so it could be used for the localization researches of other plant proteins in the future.
作者 李琰 詹晓平 杨静 刘林 李成云
机构地区 云南农业大学植物保护学院/农业生物多样性与病虫害控制教育部重点实验室
出处 《山东农业科学》 2015年第8期104-106,共3页 Shandong Agricultural Sciences
基金 国家"973"计划项目(2012CB72901)
关键词 PIN2蛋白 免疫荧光定位 水稻 根尖细胞 PIN2 protein Immunolocalization Rice Cells of root tips
分类号 S511 [农业科学—作物学] S188.2 [农业科学—农业基础科学]
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参考文献13

  • 1Galweiler L, Guan C, MUller A, et al. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue[ J]. Science, 1998, 282(5397): 2226 -2230.
  • 2Wisniewska J, Xu J, Seifertova D, et al. Polar PIN localization directs auxin flow in plants[J]. Science, 2006, 312(5775) : 883.
  • 3Grieneisen V A, Xu J, Mar:e A F, et al. Auxin transport is sufficient to generate a maximum and gradient guiding mot growth[ J]. Nature, 2007, 449 (7165) : 1008 - 1013.
  • 4Paciorek T, Zazfmalov6 E, Ruthardt N, et al. Auxin inhibits endocytosis and promotes its own efflux from cells [ J ]. Nature, 2005, 435(7046) : 1251 - 1256.
  • 5Pan J W, Fujioka S, Peng J L, et al. The E3 ubiquitin ligase SCF TIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane ac- cumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana[J]. Plant Cell, 2009, 21 (2) : 568 -580.
  • 6潘建伟,叶晓帆,王超,涂世伟.拟南芥PIN2介导的生长素极性运输调控植物根向地性[J].浙江师范大学学报（自然科学版）,2010,33(1):1-6. 被引量：12
  • 7Chen R, Hilson P, Sedbrook J, et aL The Arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar - auxin - transport efflux carrier [J]. PNAS, 1998, 95(25) : 15112 - 15117.
  • 8石江华,廖红,严小龙.植物根系向地性感应的分子机理与养分吸收[J].植物学通报,2005,22(5):523-531. 被引量：16
  • 9Brandizzi F, Fricker M, Hawes C. A greener world : the revolu- tion in plant bioimaging [ J]. Nat. Rev. Mot. Cell Biol., 2002,3(7) :520 -530.
  • 10Lauber M H, Waizengger I, Steinmann T, et al. The Arabidopsis KNOLLE protein is a cytokinesis - specific syntaxin [ J ]. J. Cell Biol. ,1997,139:1485 - 1493.

二级参考文献22

  • 1石江华,廖红,严小龙.植物根系向地性感应的分子机理与养分吸收[J].植物学通报,2005,22(5):523-531. 被引量：16
  • 2Swamp K, Benkova E, Swamp R, et al. The auxin influx cartier LAX3 promotes lateral root emergence[ J ]. Nature Cell Biology ,2008,10 (8) : 946-954.
  • 3Petrasek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting function in cellular auxin efflux [ J ]. Science, 2006,312 (5775) : 914-915.
  • 4Galweiler L, Guan Changhui, Muller A, et al. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue [ J ]. Science, 1998, 282 (5397) :2226-2230.
  • 5Wisniewska J, Xu Jian, Seifertova D, et al. Polar PIN localization directs auxin flow in plants [ J ]. Science,2006,312 (5775) : 883.
  • 6Grieneisen V A,Xu Jian, Maree A F,et al. Auxin transport is sufficient to generate a maximum and gradient guiding root growth [ J]. Nature, 2007,449(7165) :1008-1013.
  • 7Friml J, Wisniewska J, Benkova E, et al. Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis[ J]. Nature ,2002,415 (6873):806-809.
  • 8Friml J, Vieten A, Sauer M, et al. Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis [ J ]. Nature,2003,426 (6963) : 147-153.
  • 9Blilou I, Xu Jian, Wildwater M, et al. The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots [ J ]. Nature, 2005,433 ( 7021 ) :3944.
  • 10Chen Rujin, Hilson P, Sedbrook J ,et al. The Arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar-auxin-transport efflux carrier [ J ]. PNAS, 1998,95 ( 25 ) : 15112-15 ! !7.

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山东农业科学
2015年 第8期

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