摘要
60只75日龄ISA褐蛋鸡随机分成长光组(LL)和短光组(SL),试验期70 d。短光组恒定光/暗:8 h/16h;长光组起始光/暗:8 h/16 h,每周增加0.5 h,最长光/暗达到13 h/11 h。分别于105和136日龄每组取6羽蛋鸡采样,观察光周期对蛋鸡GnRH-I mRNA,GnRH-Ra mRNA表达以及卵巢发育的影响。结果表明:136 d长光组GnRH-I mRNA表达的丰度显著高于短光组(P<0.05),且极显著高于105 d长光组和短光组的表达(P<0.01)。136 d短光组GnRH-Ra mRNA表达较105 d显著下降(P<0.05),长光组GnRH-Ra mRNA的表达丰度显著高于短光组(P<0.05)。136 d长光组的卵巢重、卵巢指数极显著高于短光组(P<0.01)。以上说明,长光明显上调开产前蛋鸡下丘脑GnRH-I mRNA的表达,促进卵巢的发育;短光则明显下调垂体GnRH-Ra mRNA的表达,致使卵巢发育延缓。
60 ISA brown layers at 75 day-old were randomly divided into long light group（ LL） and short light group（ SL）,the experiment lasted 70 days. SL constant light / dark： 8 h/16 h; LL set of initial light / dark： 8 h/16 h,A weekly increase of 0. 5 h,the light / dark reaches 13 h /11 h. Six layers were selected randomly in each group at 105-day-old and 136-day-old respectively to observe the effects of photoperiods on GnRH-I mRNA,GnRH-Ra mRNA expres-sion and ovarian development in layers. The results indicated that GnRH-I mRNA expression abundance in LL was significantly higher than that of SL（ P 0. 05） the age at the136-day-old,and significantly higher than that of LL and SL（ P 0. 01） at 105-day-old. The GnRH-Ra mRNA expression in SL at 136-day-old was significantly decreased than that of 105-day-old（ P 0. 05）,the expression abundance of GnRH-Ra mRNA in LL was significantly higher than that of SL（ P 0. 05）. At the136-day-old,ovary weight,ovary index in LL was significantly higher than that of SL（ P 0. 01）.Therefore,the long photoperiods obviously up-regulated GnRH-I mRNA expression in layers ＇ hypothalamus before start laying,to promote the ovarian development,the short photoperiods significant down-regulation GnRH-Ra mRNA expression in pituitary and cause delay of ovarian development.
出处
《激光生物学报》
CAS
2015年第3期251-256,共6页
Acta Laser Biology Sinica
基金
安徽科技学院重点学科基金项目(AKXK20101-2)
安徽蚌埠市农业科技计划项目(2013)9
安徽蚌埠市3221产业创新团队工程(12010147)
