乳腺癌手术前后血清中可溶性E-钙黏连蛋白的表达及临床意义

Expression and Clinical Significance of Soluble E-cadherin before and after Operation in Breast Cancer Patients

目的检测乳腺癌患者手术治疗前后血清中可溶性E-钙黏连蛋白(EC)的表达并探讨其临床意义。方法采用酶联免疫吸附法(ELISA)检测42例乳腺癌患者手术前后、18例乳腺良性病变患者手术前后以及30例健康人血清中可溶性EC的表达水平。并分析乳腺癌手术前后血清中可溶性EC的水平变化及与其临床病理特征的关系。结果乳腺癌患者术前血清可溶性EC较乳腺良性病变患者和健康人显著升高;乳腺癌患者术后血清可溶性EC水平较术前明显下降;高分期以及伴有淋巴结转移的乳腺癌患者的血清可溶性EC明显高于低分期及无淋巴结转移患者(P<0.05)。结论乳腺癌患者血清可溶性EC在手术前后的表达具有特异性并且与肿瘤病理分期以及淋巴结转移显著相关,可能成为乳腺癌患者预后的预测因素。

Objective To detect the expression of serum soluble E-cadherin（s EC）before and after operation in breast cancer patients,and to explore its clinical significance. Methods The levels of s EC in 42 patients with breast cancer and 18 patients with benign breast diseases were tested by enzyme-linked immunosorbent assay（ELISA）before and after operation as well as in 30 healthy controls. Its correlation with clinical and pathological characteristics was analyzed. Results The levels of s EC in breast cancer patients before operation were significantly higher than patients with benign breast diseases and controls,which declined significantly after operation. Moreover,the levels of s EC in those with high clinical grading and lymphatic metastasis were also higher than in the ones with low grading and negative lymphatic metastasis（P〈0.05）. Conclusion The levels of s EC in breast cancer patients before operation are specific and closely related with pathological grade and lymphatic metastasis,which may be a predictive factor for prognosis of breast cancer.