独一味的HPLC指纹图谱研究

HPLC fingerprint of Lamiophlomis rotata

目的：建立独一味的指纹图谱分析方法用于该药材的质量评价。方法：采用HPLC法,选用Welchrom C18色谱柱（250mm×4.6 mm,5μm）,以乙腈（A）-0.1%磷酸水溶液（B）为流动相,梯度洗脱（0~10 min,5%A→8%A;10~27 min,8%A→12%A;27~35 min,12%A;35~70 min,12%A→17%A;70~80 min,17%A→19%A;80~110 min,19%A→36%A;110~120min,36%A→50%A）,流速1.0 m L·min^-1,检测波长235 nm,柱温30℃;用国家药典委员会＂中药色谱指纹图谱相似度评价系统2004A＂处理分析。结果：对23批独一味地上部分样品指纹图谱进行评价,确定了14个共有峰,指认了第3、4、5、8、9、10、11、12号色谱峰分别对应为胡麻属苷、山栀苷甲酯、绿原酸、马钱苷、8-O-乙酰山栀苷甲酯、连翘酯苷B、木犀草苷和麦角甾苷。结论：本研究可更全面、准确地评价独一味药材质量,为该药材的质量控制提供科学依据。

Objective： To establish a fingerprint analysis method for quality evaluation of Lamiophlomis rotata.Methods： HPLC was employed,and the separation was performed on a Welchrom- C18 chromatographic column（ 250 mm × 4. 6 mm,5 μm） using acetonitrile（ A）- 0. 1 % orthophosphoric acid（ B） as mobile phase with gradient elution（ 0-10 min,5 % A→8 % A; 10-27 min,8 % A→12 % A; 27-35 min,12 % A; 35-70 min,12 % A→17 % A; 70-80 min,17 % A →19 % A; 80-110 min,19 % A →36 % A; 110-120 min,36 % A →50 %A） at a flow rate of 1. 0 m L·min^-1. The detection wavelength was set at 235 nm and the column temperature was controlled at 30 ℃. The data were analyzed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine（ 2004A）. Results： The total 23 batches of aerial part of Lamiophlomis rotata were evaluated,and fourteen common chromatographic peaks were observed. Then,the No. 3,No. 4,No. 5,No. 8,No. 9,No.10,No. 11 and No. 12 peaks were identified as sesamoside,shanzhiside methylester,chlorogenic acid,loganin,8-Oacetyl shanzhiside methylester,forsythiaside B,luteolin glycosides and acteoside,respectively. Conclusion： The established method is more comprehensive and accurate in evaluating the quality of Lamiophlomis Herba and can provide the scientific basis for quality control.