基于“有故无殒”思想的醋甘遂毒性研究

Study on toxicity of vinegar-processed Kansui Radix on basis of symptom-based prescription theory

目的:研究醋甘遂对正常和癌性腹水模型大鼠毒性的差异。方法:以正常和癌性腹水模型大鼠为研究对象,分组连续7 d灌胃不同剂量醋甘遂,通过病理切片观察大鼠肝、胃、肠组织损伤情况,并考察其对大鼠血清、肝、胃、肠组织生化指标及氧化损伤指标的影响。结果:与空白组比较,各正常给药组及模型组大鼠肝、胃、肠组织均出现显著损伤。与模型组比较,各模型给药组损伤显著减轻。与空白组比较,模型组大鼠血清、肝脏中谷草转氨酶(AST)、谷丙转氨酶(ALT)活力显著升高(P<0.01),血清及肝、胃、肠组织中谷胱甘肽(GSH)含量显著降低(P<0.01),丙二醛(MDA)含量显著升高(P<0.01)。与空白组比较,正常低、中、高剂量组大鼠血清中AST,ALT与肝脏中ALT及正常高剂量组大鼠肝脏中AST活力均显著增高(P<0.05,P<0.01);正常低、中、高剂量组血清、胃组织中GSH及正常中、高剂量组肝、肠组织中GSH含量显著降低(P<0.05,P<0.01);正常低、中、高剂量组肝组织中MDA及正常中、高剂量组血清、胃、肠组织中MDA含量显著升高(P<0.05,P<0.01)。与模型组比较,模型低、中、高剂量组大鼠血清中AST,ALT与模型中、高剂量组大鼠肝脏中AST及模型高剂量组大鼠肝脏中ALT活力均显著降低(P<0.05,P<0.01);模型低、中、高剂量组血清及胃组织与模型中、高剂量组肝组织及模型高剂量组肠组织中GSH含量显著升高(P<0.05,P<0.01);模型低、中、高剂量组血清与模型中、高剂量组肝组织及模型高剂量组胃、肠组织中MDA含量显著降低(P<0.05,P<0.01)。结论:醋甘遂对癌性腹水模型大鼠肝、胃、肠毒性较正常大鼠显著降低,为醋甘遂"有故无殒"的临床安全用药提供依据。

Objective： To study the differences in the toxicity of vinegar-processed Kansui Radix on normal and cancerous ascites model rats. Method： Normal and cancerous ascites model rats were taken as the research objects and orally administered with different doses of vinegar-processed Kansui Radix for 7 d. Pathological sections were prepared to observe the damages in liver, stomach, intesti- nal tissues in rats and detect the impacts on serum, liver, stomach and intestinal tissues and the oxidative damage index. Result： Com- pared with the blank group, all of normal administration groups and model groups showed significant damages in liver, stomach and in- testinal tissues. Compared with the model groups, all of normal administration groups revealed notable alleviation in damages. Com- pared with the blank group, the model groups showed significant increases in AST, ALT and MDA in serum and liver （P 〈 0. 01 ） and a significant decrease in GSH in serum and liver, stomach, intestinal tissues （P 〈 0.01 ）. Compared with the blank group, the resultsshowed significant decreases in ALT, AST in serum and ALT in liver in model low, medium and high dose groups and AST activity in liver tissues in the normal high dose group （P 〈0. 05, P 〈0. 01 ） ; significant decreases in GSH in serum and stomach tissues in nor- mal low, medium and high dose groups and GSH content in liver and intestinal tissues in normal medium and high dose groups （ P 〈 0. 05, P 〈0.01 ） ; notable rises in MDA in liver tissues in normal low, medium and high dose groups and MDA content in serum and stomach and intestinal tissues in normal medium and high dose groups （P 〈0. 05, P 〈0.01 ）. Compared with model groups, data re- vealed significant decreases in ALT, AST in serum in model low, medium and high dose groups, AST in liver tissues of model medium and high dose groups and ALT activity in liver in the model high dose group （P 〈0.05, P 〈0. 01 ） ; significant increases in GSH con- tent in serum and stomach tissues of model low, medium and high dose groups, GSH in liver tissues in model medium and high dose groups and GSH in intestinal tissues in the high dose groups （P 〈 0. 05, P 〈 0. 01 ） ; and notable declines in MDA content in serum in model low, medium and high dose groups, MDA in liver tissues of model medium and high dose groups and MDA in stomach and intes- tinal tissues the high dose group （P 〈 0.05, P 〈 0.01 ）. Conclusion： According to the study, vinegar-processed Kansui Radix showed a significant lower toxicity liver, stomach, and intestines of cancerous ascites model rats, which provided a basis for clinical safe appli- cation of vinegar-processed Kansui Radix based on symptom-based prescription theory.