富血小板血浆对老龄犬纤维多孔钛颈椎椎间融合器沉降的影响

Effects of platelet-rich plasma on subsidence of fiber porous titanium Cage for old dogs with anterior cervical interbody fusion

目的观察富血小板血浆（PRP）对老龄比格犬颈椎前路椎间融合术纤维多孔钛椎间融合器（FPT Cage）沉降的影响。方法选取6只7~9岁、12.5~15.0 kg的健康比格犬（雌雄各3只）作为实验动物。取比格犬前肢外周静脉血,采用二次离心法制备PRP,同时对外周静脉血及PRP进行血小板计数。行颈椎前路C4/5、C5/6椎间盘切除、FPT Cage椎间融合术,单纯使用未浸泡PRP的FPT Cage行C4/5椎间融合,采用浸泡激活后的PRP复合FPT Cage融合C5/6,术后4个月处死实验犬后行X线片及Micro-CT检查,观察C4/5、C5/6椎间融合器的沉降情况。结果 C4/5节段有4例出现不同程度的椎间融合器沉降,另2例出现Cage向前移位;C5/6节段未有椎间融合器发生沉降,仅1例出现Cage向前移位。结论复合PRP可减少老龄比格犬颈椎椎间融合术FPT Cage的沉降。

Objective To observe the effects of platelet-rich plasma（PRP） on subsidence of fiber porous titanium（FPT） Cage for old beagle dogs with anterior cervical interbody fusion. Methods Six old healthy beagle dogs（3 male and 3 female, weighing 12.5-15.0 kg, aged 7-9 years） were selected in this study. Peripheral venous blood from dog＇s forelimb was collected, PRP was prepared by double centrifugal methods, and blood platelet counts of peripheral venous blood and PRP were recorded. Then the dogs underwent anterior C4/5 and C5/6 discectomy and FPT Cage interbody fusion. FPT Cage was used alone at C4/5 segment, and at C5/6segment, FPT Cage was augmented with PRP. The animals were sacrificed in 4 months after the surgery, and the subsidence of FPT Cage was evaluated by X-ray and Micro-CT examinations. Results Subsidence of FPT Cage was found at four C4/5 segments, other two FPT Cages at C4/5 segment showed anterior migration; No subsidence of FPT Cage was observed at six C5/6 segments, only one FPT Cage with anterior dislocation was found. Conclusion Compound PRP could reduce the subsidence of FPT Cage in old aged Beagle dogs with anterior cervical interbody fusion.