载辛伐他汀PLGA/CPC支架材料复合BMSCs修复大鼠颅骨缺损的实验研究

Effect of Simvastatin Carried by PLGA/CPC Combining with BMSCs on the Rat Critical Calvarial Defect

目的:探讨载辛伐他汀PLGA/CPC支架材料复合骨髓间充质干细胞(BMSCs)构建组织工程骨,修复大鼠颅骨临界尺寸骨缺损的可行性以及效果。方法:24只雄性大鼠随机均分为3组;在大鼠颅骨人字缝两侧各做一直径5mm的骨缺损。每组16个骨缺损再随机分为:载辛伐他汀PLGA/CPC/BMSCs(Brdu标记的BMSCs)组(n=4);载辛伐他汀PLGA/CPC组(n=4);单纯PLGA/CPC组(n=4)以及空白对照组(n=4)。术后4、8、12周取标本分别进行大体观察,HE染色及免疫组织化学染色来评价骨再生情况。结果:术后4、8、12周组织学观察表明载辛伐他汀PLGA/CPC/BMSCs组的成骨质量和速度明显优于其他3组,免疫组化结果显示载辛伐他汀PLGA/CPC/BMSCs组骨钙素(OC)阳性表达IOD值明显高于其他3组。结论:载辛伐他汀PLGA/CPC支架材料复合BMSCs可以诱导大鼠颅骨临界尺寸骨缺损内的新骨形成,成骨质量优于其他3组,而且可以明显缩短骨愈合时间。

Objective：To investigate the possibilities and restoration effect of simvastatin carried by PLGA/CPC combining with BMSCs for reconstructing tissue-engineering bone in the calvarias of rats with critical sized defects.Methods：A total of 24 male rats were divided into three groups randomly with 8in each group.Two circular 5mm diameter defects were prepared on both sides of rat calvarial bone.The 16 calvarial bone defects in each group were randomly divided as simvastatin/PLGA/CPC/BMSCs group（BMSCs signed by Brdu）（n=4）,simvastatin/PLGA/CPC group（n=4）,PLGA/CPC group（n=4）,and blank control group（n=4）.After 4,8,12 weeks post-operation,all specimens were assessed using general observation,HE,and immunohistochemistry methods to evaluate the bone regeneration.Results：After 4,8and 12 weeks post-operation,histological results showed that the bone quality and bone formation quantity at the group with simvastatin/PLGA/CPC/BMSCs were much better than those of other groups.The results of immunohistochemistry showed that the positive expression of osteocalcin at simvastatin/PLGA/CPC/BMSCs group was much higher than that of other three groups.Conclusion：Simvastatin PLGA/CPC scaffold materials combined with BMSCs can induce new bone formation in critical sized calvarial defect,form high quality bone,and shorten the bone healing time when compared with other three groups.