三七总皂苷对糖尿病肾病大鼠肾功能的作用研究

Effect of Panax Notoginsenosidum on the renal function in diabetic nephropathy rats

目的观察三七总皂苷(Panax Notoginsenosidum,PNS)对糖尿病肾病(diabetic nephropathy,DN)大鼠肾功能的影响,探讨PNS防治DN的作用及机制。方法 48只SD大鼠随机分为正常组、模型组、依那普利组、PNS干预组,每组各12只,除正常组外,其余3组大鼠在成功建立DN模型后,分别给予生理盐水、依那普利和PNS灌胃治疗,1次/d;10周后,检测4组大鼠血清三酰甘油(triacylglycerol,TG)、总胆固醇(total cholesterol,TC)、血清肌酐(serum creatinine,SCr)、血清尿素氮(blood urea nitrogen,BUN)水平,以及尿蛋白(urine protein,UP)和肾脏晚期糖基化终末产物(advanced glycation endoproducts,AGEs)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)水平。结果模型组大鼠血清TG、TC、SCr、BUN、UP、AGEs、MDA均高于正常组(P<0.05),而肾脏SOD水平低于正常组(P<0.05);PNS干预组血清TG[(1.15±0.30)mmol/L]、TC[(2.43±0.46)mmol/L]、SCr[(69.58±5.62)μmol/L]、BUN[(18.28±2.02)mmol/L]、UP[(7.23±2.58)mg/24h]和AGEs[(27.18±2.81)AUF/mg]、MDA[(8.92±0.80)nmol/mg]较模型组[TG(1.61±0.37)mmol/L、TC(3.92±0.58)mmol/L、SCr(92.83±6.78)μmol/L、BUN(21.04±3.66)mmol/L、UP(19.37±10.50)mg/24 h、AGEs(38.67±4.08)AUF/mg、MDA(15.17±1.26)nmol/mg]明显降低(P<0.05),而SOD[(30.26±2.09)u/mg]水平较模型组[(14.89±1.15)u/mg]明显升高(P<0.05);PNS组与依那普利组以上指标比较差异均无统计学意义(P>0.05)。结论 PNS可能通过抗氧化、抑制AGEs生成、降低UP、TG、TC、SCr、BUN水平,减轻DN的肾脏损害,对DN大鼠肾功能起保护作用。

Objective To observe the effect of Panax Notoginsenosidum （PNS） on the prevention and therapy of diabetic nephropathy （DN） and its mechanism by observing its influence on the renal function in DN rats. Methods Forty-eight male rats were randomly divided into normal group, model group, enalapril group and PNS group, with 12 rats in each group. Except control group, the other three groups received gavage with normal saline, enalapril and PNS respectively （once per day） after the establishment of DN models. After 10 weeks, all these four groups were observed the levels of serum triacylglycerol （TG）, serum total cholesterol （TC）, serum creatinine （SCr）, blood urea nitrogen （BUN）, urine protein （UP）, renal advanced glycation endoproducts （AGEs）, renal superoxide dismutase （SOD） and renal malondialdehyde （MDA）. Results The levels of TG, TC, SCr, BUN, UP, AGEs and MDA were significantly higher and SOD level was significantly lower in model group than those in normal group （P〈0.05）. The levels of TG （（1.15 ± 0.30） mmol/L）, TC （（2.43±0.46） mmol/L）, SCr （（69.58±5. 62） μmol/L）, BUN （（18.28±2.02） mmol/L）, UP （（7.23±2.58） mg/24 h）, AGEs （（27. 18 ± 2.81） AUF/mg） and MDA （（8. 92± 0. 80） nmol/mg） were significantly lower in PNS group than those in model group （TG： （1. 61 ± 0. 37）mmol/L, TC： （3. 92±0. 58） mmol/L, SCr： （92.83±6.78）μmol/L, BUN： （21.04±3.66） mmol/L, UP： （19.37±10.50） mg/24 h, AGEs： （38.67±4.08） AUF/ mg, MDA： （15.17±1.26） nmol/mg） （P（0.05）, and SOD was higher in PNS group （（30.26±2.09） u/mg） than that in model group （（14.89±1.15） u/mg） （P〈0.05）. There were no significant differences in the above indexes between PNS group and enalapril group （P〉0.05）. Conclusion PNS might reduce kidney damage in DN by anti-oxidant, inhibiting overproduction of AGEs, and reducing the levels of UP, TG, TC, SCr and BUN, and further protect the renal function in DN rats.