摘要
The utilization and speciation transformation of α-S8 and μ-S by the typical mesophilic acidophilic strain Acidithiobacillus ferrooxidans ATCC 23270 were investigated.A.ferrooxidans cells first acclimated to the energy source α-S8 or μ-S,respectively.The results of cell growth and sulfur oxidation behavior showed that the strain grown on α-S8 entered slowly(about 1 d later) into the exponential phase,while grew faster in the exponential phase and attained higher maximal cell density and lower p H value than that on μ-S.After bio-corrosion,both of the two sulfur samples were evidently eroded and modified by A.ferrooxidans cells.After growth of A.ferrooxidans,the surface composition of amorphous μ-S became 63.1% μ-S and 36.9% α-S8,and that of orthorhombic α-S8 became 68.3% α-S8 and 31.7% μ-S,while the surface compositions of α-S8 and μ-S in sterile experiment were not changed,indicating that these two elemental sulfur species can be interconverted by A.ferrooxidans.
研究典型中温嗜酸菌株Acidithiobacillus ferrooxidans ATCC 23270对两种不同形态单质硫(α-S8和μ-S)的利用和硫形态转化。A.ferrooxidans分别在α-S8或者μ-S中培养以适应能源底物。A.ferrooxidans在这两种单质硫中的生长和硫氧化行为的结果表明,A.ferrooxidans在α-S8中生长时比在μ-S中生长晚1 d进入对数期,但是A.ferrooxidans在α-S8中生长时在对数期生长较快且培养液具有较高的细胞浓度和较低的p H值。经过A.ferrooxidans的培养,这两种单质硫都被明显腐蚀和细胞修饰。此外,经过A.ferrooxidans的生长,无定型态的μ-S表面组分变为63.1%μ-S和36.9%α-S8,正交晶系的α-S8表面组分变为68.3%α-S8和31.7%μ-S;而无菌对照组μ-S和α-S8表面组分都没有变化,表明这两种不同形态的单质硫在A.ferrooxidans的作用下发生了相互转化。
基金
Project(51274257)supported by the National Natural Science Foundation of China
Project(U1232103)supported by the Joint Funds of National Natural Science Foundation of China and Large Scientific Facility Foundation of Chinese Academy of Sciences
Project(VR-12419)supported by the Beijing Synchrotron Radiation Facility Public User Program