糖络宁对STZ诱导DPN大鼠氧化应激的影响

The Effects of Tangluoning on Oxidative Stress of Diabetic Peripheral Neuropathy Rats

目的观察糖络宁对链脲佐菌素(STZ)诱导糖尿病周围神经病变(DPN)大鼠氧化应激的影响。方法SD大鼠60只,随机选取10只为正常对照组,其余50只大鼠一次性腹腔注射60 mg/kg STZ建立糖尿病大鼠模型,造模成功后随机分为模型对照组、α-硫辛酸组、糖络宁低剂量组和糖络宁高剂量组。药物干预12周后,检测大鼠血清中总抗氧化能力(T-AOC)及抗氧化酶如谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等的活力,同时测定氧化应激产物如丙二醛(MDA)含量、活性氧自由基(ROS)和诱导型一氧化氮合酶(iNOS)的活力。结果与空白对照组比较,模型对照组T-AOC降低(P<0.01),抗氧化酶GSH-Px、SOD、CAT活力降低(P<0.01);氧化应激产物MDA、ROS含量升高(P<0.01),iNOS的活力升高(P<0.01)。与模型对照组比较,糖络宁低剂量组T-AOC升高(P<0.01),抗氧化酶GSH-Px、SOD、CAT活力升高(P<0.01);氧化应激产物MDA含量降低(P<0.01),ROS含量降低(P<0.05),iNOS的活力降低(P<0.01)。糖络宁高剂量组T-AOC的活力升高(P<0.05),CAT的活力升高(P<0.01);氧化应激产物MDA含量降低(P<0.01),ROS含量降低(P<0.05),iNOS的活力降低(P<0.01)。结论中药复方糖络宁可以明显提高具有抗氧化能力物质SOD、GPx、CAT含量和T-AOC的活力,清除对周围神经有害的氧化代谢产物及氧化应激活性物质MDA、ROS和iNOS的含量及活力,具有明显抗氧化应激能力,减轻氧化损伤,对糖尿病周围神经病变(DPN)有明显的保护作用。

Objective： To investigate the effects of Tangluoning on oxidative stress of diabetic peripheral neu- ropathy rats. Methods： 60 SD rats were randomly divided into the control group with 10 cases and the experi- mental group with 50 cases. Rats in the experimental group were intraperitoneal injected 60 mg/kg STZ to induce diabetic rat model. Rats with fasting blood glucose concentration greater than or equal to 16.7 mmol/L after 72 h were treated as diabetes and were randomly divided into the model group, ct-lipoic acid group （ALA）,low dose of Tang Luo Ning group （LTLN） and high dose of Tang Luo Ning group （HTLN）. After treated with Tang Luo Ning for 12 weeks,T-AOC,GSH-Px,SOD,CAT in serum were detected,and at the same time,the content of MDA, ROS and the activity of iNOS in serum were measured. Results： The data showed that compared with the control group,T-AOC, GSH-Px,SOD, CAT in the model group markably decreased （P〈 0.01 ）,while the content of MDA and ROS and the activity of iNOS increased （P〈 0.01 ）. Compared with the model group,T-AOC ,GSH-Px,SOD, CAT of LTLN treated group increased（P〈 0.01 ） ,while the content of MDA and ROS and the activity of iNOS de- creased（P〈 0.01 ）. Compared with the model group T-AOC,CAT of HTLN treated group increased（P〈 0.05 or P〈 0.01） and the content of MDA and ROS and the activity of iNOS decreased （P〈 0.05 or P〈 0.01 ）. Conclusion： Tangluoning can increase the vitality of GSH-Px, SOD, CAT and T-AOC, clear the content of MDA and ROS and decrease the vitality of iNOS with TLN prevented diabetic peripheral neuropathy by attenuating oxidative stressinjury.