摘要
微型反向重复转座基因(minialure inverted repeat transposable elements,MITEs)的演化可能是造成枣树基因组及品种多样性的重要原因.为研究MITEs在枣树全基因组中的分布、种类及演化,使用MITE预测软件(MITEDigger)识别枣树全基因组中的MITEs序列,在植物MITEs数据库中进行分类注释,并用MEGA 6.0构建枣树基因组中MITEs的系统进化树.结果显示,MITE-Digger检测出MITEs总共3 230条,碱基数为1 104 679 bp,占基因组比例为0.34%;其中共有948条在植物MITEs数据库中比对到相似序列,这些MITEs可以归类到5个家族:h AT,PIF/Harbinger,CACTA,Mutator和Tc1/Mariner,MITEs系统进化树显示:枣树基因组中MITEs来源于少数几个共同祖先,在长期的进化中有明显的扩增和突变,这可能与枣树品种多样性有关.研究结果将为枣树的遗传资源及育种研究提供重要参考.
The evolution of the MITEs might be the important cause of Chinese jujube genome and species diversity. In order to explore the distribution,types and evolution of MITEs in jujube genome,MITE prediction software( MITE-Digger) was used to identify the MITEs sequence of jujube genome,annotated in plant MITEs classified database. The phylogenetic tree of MITEs in jujube genome was built up with MEGA 6. 0. The results indicated that the MITE-Digger detected 3 230 pieces of MITE,with 1 104 679 bp bases which account for 0. 34% of the whole genome. The similar sequence compared had 948 pieces of MITEs in the plant MITEs database( P-MITE). These MITEs could be categorized into five families:h AT,PIF / Harbinger,CACTA,Mutator and Tc1 / Mariner. MITEs phylogenetic tree showed that MITEs of jujube genome came from a few common ancestor,and had no obvious amplification and mutation in the long-term evolution,which might be related to the diversity of jujube varieties. The findings could provide important reference for genetic resources and breeding of Ziziphus Jujuba.
出处
《郑州大学学报(理学版)》
CAS
北大核心
2015年第4期103-107,共5页
Journal of Zhengzhou University:Natural Science Edition
基金
NSFC-河南人才培养联合基金资助项目
编号U1204307
河南省高等学校重点科研项目
编号15A2100410
洛阳师范学院省级培育项目
编号2010-PYJJ-007