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Screening and Application of Molecular Markers for Starch Synthesis-related Genes in Rice

水稻淀粉合成相关基因分子标记的筛选与利用(英文)
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摘要 In this study, the genotypes of starch synthesis-related genes were systematically screened from different rice varieties using molecular markers. The results showed that starch synthesis-related genes were highly polymorphic between indica and japonica varieties, as they greatly variated among indica varieties, but were conserved among japonica varieties. The genotypes of two indica varieties9311 and Minghui 63 were more similar to that of japonica varieties. Two or three alleles of six starch synthesis-related genes were found in 28 japonica parental varieties. Four genotypes of two soluble starch synthase genes, SSIIa and SSIIIa,were detected in 88 stable lines derived from the cross of Kanto 194/ Wujing 13 using molecular markers. 利用分子标记检测的方法,对不同水稻品种间的淀粉合成相关基因的基因型进行了系统筛选。结果显示,淀粉合成相关基因在籼粳亚种间表现出较好的多态性,在籼稻品种内存在较大变异,但在粳稻品种内较为保守,籼稻品种9311和明恢63的基因型更接近于粳稻品种;在28个粳稻亲本中检测到6个淀粉合成相关基因存在着2种或3种等位基因型。利用在关东194和武粳13之间具有多态性的可溶性淀粉合酶基因SSIIa和SSIIIa的分子标记,在其杂交组合衍生的88份粳稻品系中检测到2个基因的4种基因型。
出处 《Agricultural Science & Technology》 CAS 2016年第1期29-33,共5页 农业科学与技术(英文版)
基金 Supported by the Agricultural Science Independent Innovation Foundation of Jiangsu Province[C X(12)1003] Key Technology Research and Development Program of Jiangsu Province(BE2013301) Earmarked Fund for China Agriculture Research System(CARS-01-47)~~
关键词 Rice(Oryza sativa L Starch synthesis-related gene Molecular marker High quality 水稻 淀粉合成相关基因 分子标记 优质育种
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