ASGPR与HBV preS1蛋白之间相互作用的验证

Verification of the interaction between ASGPR and HBV preS1 protein

目的验证去唾液酸糖蛋白受体(asialoglycoprotein receptor,ASGPR)与乙肝病毒前S1蛋白(HBV preS1蛋白)之间的相互作用,确认ASGPR作为乙肝病毒肝细胞膜受体在介导乙肝病毒感染的分子机制中的作用。方法分别用哺乳动物双杂交及体外免疫共沉淀技术验证ASGPR与HBV preS1蛋白之间的相互作用,操作方法参照试剂盒说明书进行。结果哺乳动物双杂交实验结果提示,ASGPR与HBV preS1蛋白在细胞环境中具有相互作用;免疫共沉淀实验结果提示,ASGPR与HBV preS1蛋白在非细胞环境中具有相互作用。结论 ASGPR可能是介导HBV入侵的肝细胞膜受体之一。

Objective To verify the interaction between asialoglycoprotein receptor （ASGPR） and hcpatitis B virus （HBV） preS1 protein in vivo and in vitro, and identify ASGPR as a cell-surface receptor for HBV, which could elucidate the molecular mechanism of HBV infection. Methods The preS1-ASGPR interaction was examined in mammalian two.hybrid and coimmunoprecipitation system by strictly following the manufacturer＇s instructions. Results ASGPR interacted specifically and directly with the preS1 domain of HBV in vivo and its vitro. Conclusion ASGPR may be a candidate receptor for HBV that mediates further step of HBV entry.