HPLC波长转换法同时测定天麻头痛片中天麻素、欧前胡素、阿魏酸、胡薄荷酮和11-羰基-β-乙酰乳香酸的含量

Simultaneous Determination of Gastrodin, Imperatorin, Ferulic Acid, Pulegone and Acetyl-11-keto-β-boswellic Acid in Tianma Toutong Tablets by Wavelength Conversion HPLC

目的建立同时测定天麻头痛片中天麻素、欧前胡素、阿魏酸、胡薄荷酮和11-羰基-β-乙酰乳香酸含量的HPLC方法。方法采用Agilent Zorbax TC-C18色谱柱,流动相为乙腈-0.5%磷酸溶液,流速为1.2 m L·min？1,梯度洗脱,进样量为10μL,柱温为35℃。结果天麻素、欧前胡素、阿魏酸、胡薄荷酮和11-羰基-β-乙酰乳香酸检测浓度分别在1.25～25.00,0.50～10.00,10.00～200.00,0.50～10.00,0.50～10.00μg·m L-1内与峰面积积分值呈良好的线性关系;精密度、稳定性、重复性试验的RSD均≤1.0%;平均加样回收率分别为100.0%,99.4%,99.1%,98.9%,98.6%,RSD分别为1.33%,1.38%,0.79%,3.12%,1.26%（n=9）。结论该方法简便、准确、重复性好,可为天麻头痛片的质量控制提供实验依据。

OBJECTIVE To develop a method for the content determination of gastrodin, imperatorin, ferulic acid, pulegone and acetyl-11-keto-β-boswellic acid in Tianma Toutong tablets simultaneously by HPLC. METHODS The determination was performed on Agilent Zorbax TC-C18 column with mobile phase consisted of actonitrile-0.5% phosphoric acid solution at the flow rate of 1.2 m L·min-1, gradient elution and the injection volum was 10 μL. The column temperature was 35 ℃. RESULTS The linear ranges of gastrodin, imperatorin, ferulic acid, pulegone and acetyl-11-keto-β-boswellic were 1.25-25.00, 0.50-10.00, 10.00-200.00, 0.50-10.00 and 0.50-10.00 μg·min-1, respectively; RSDs of precision, stability and reproducibility tests were all ≤1.0%. The average recoveries were 100.0%, 99.4%, 99.1%, 98.9%, 98.6%; RSDs were 1.33%, 1.38%, 0.79%, 3.12%, 1.26%（n=9）, respectively. CONCLUSION The method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of Tianma Toutong tablets.