早衰小鼠骨髓间充质干细胞电压门控钙通道的表达检测

Voltage-gated calcium channel expression in BMMSCs of progeria mice and type mice

目的:研究早衰小鼠骨髓间充质干细胞(BMMSCs)的成骨分化特点以及电压门控钙通道的表达特点。方法:用micro-CT检测zmpste24-/-早衰小鼠和野生小鼠的骨量差异;分离培养两者的BMMSCs,并取第2代细胞分别检测其成骨能力以及胞内钙离子的浓度,采用RT-PCR方法检测两种小鼠BMMSCs中电压门控钙通道(10种亚型)的表达水平。结果:与野生型小鼠相比,zmpste24-/-早衰小鼠的骨量、其BMMSCs的成骨分化能力和胞内钙离子浓度均明显降低(P<0.05);RT-PCR检测结果显示,电压门控钙离子通道的10种亚型在两者BMMSCs中均有表达,其中Cav1.1、Cav1.2、Cav2.1和Cav2.2在早衰小鼠BMMSCs中的表达水平均明显低于野生小鼠组(P<0.05)。结论:在早衰过程中,电压门控钙离子通道可能参与了BMMSCs介导的骨质疏松。

AIM： To investigate the characteristics of BMMSC osteogenic differentiation and the expression of voltage- gated calcium channel（ VGCC） in progeria mice. METHODS： The bone mass of zmpste24^- /-mice and wild type（ WT） mice were examined by micro- CT. BMMSCs from both types of mice were isolated and cultured. The osteogenesis,intracellular calcium ion concentration level and the mRNA expression of VGCC of the second passage BMMSCs were measured and compared. RESULTS： Compare with WT mice,bone mass of zmpste24^- /-mice was reduced. Osteogenic ability and intracellular calcium ion concentration of BMMSCs were significantly reduced in zmpste24^- /-mice. PCR results showed that the 10 sub- types of VGCC were expressed in BMMSCs of both mice,and Cav1. 1,1. 2,2. 1 and 2. 2 were down regulated in zmpste24^- /-mice. CONCLUSION： VGCC might be involved in BMMSCs-mediated osteoporosis process.