微/纳米化载锶涂层对骨髓间充质干细胞生物活性影响的研究

The effects of strontium micro / nano- scale coating on the biological activity of BMMSCs

目的:评价微/纳米化载锶涂层对骨髓间充质干细胞(BMMSCs)生物活性的影响。方法:将纯钛片分为3组,A组:光滑组(未经任何处理,n=24);B组:氢氟酸(HF)酸蚀组(n=24);C组:HF酸蚀+磁控溅射组(n=27)。SEM观察钛片表面形貌;X射线能谱(EDS)分析其表面元素含量;表面接触角检测钛片表面亲水性;离子释放试验检测C组的锶离子释放情况。在3组钛片表面分别接种BMMSCs,观察BMMSCs早期粘附能力;MTT检测细胞增殖能力;ALP活性评估细胞成骨分化能力。结果:3组钛片经不同方法处理后,B组形成微米级表面形貌;C组形成微/纳米表面形貌,并载入了锶元素,且锶元素可以离子形式释放;B、C组的亲水性、细胞粘附及增殖能力均高于A组,且B组高于C组;C组表面细胞的ALP活性显著高于B组。结论:微/纳米化载锶涂层有助于促进BMMSCs的增殖和成骨分化能力。

AIM： To evaluate the effects of strontium micro/nano scale coating on the biological activity of BMMSCs. METHODS： Titanium plates were divided into 3 groups： polished surface group（ n = 24,group A）,Hydrofluoric（ HF） acid etched surface group（ n = 24,group B）,and HF acid etched plus magnetron sputtered surface group（ n = 27,group C）. The surface properties of the plates were observed by SEM. The elemental composition was assessed by an energy dispersive X- Ray spectroscopy detector（ EDS）. The hydrophilia was evaluated by contact angles analysis. Sr2＋release was examined. BMMSCs were seeded onto the surface of the plates,early adhesion and morphology of BMMSCs were observed by confocal microscopy and SEM. Cell viability and ALP activity were detected by MTT assay and ALP activity detection kits respectively. RESULTS： Micro-scale surface structure was formed after HF etching or after HF acid etched plus magnetron sputtering. The contact angle of the plate surface of group B and C was smaller than that of group A. The strontium was loaded on the samlde surface and could be released in group C.The cells in group B and C showed higher adhesion and proliferation than those in group A. However,Group C showed the highest ALP activity among the 3 groups. CONCLUSION： The strontium- coated micro / nano scale surface can promote the proliferation and osteogenic differentiation of BMMSCs.