绵羊ACSL1基因cDNA序列克隆及其序列分析

Cloning and Sequence Analysis of ACSL1 Gene in Sheep

为研究绵羊长链脂酰辅酶A合成酶1(Long-chain fatty acyl-Co A 1,ACSL1)基因功能,以绵羊肝组织总RNA为模板,应用RACE技术扩增得到了绵羊ACSL1基因完整的CDS区以及3'端序列,利用生物信息学软件对已知序列及其预测蛋白结构进行了分析。结果表明:绵羊ACSL1基因序列开放阅读框长为2 100 bp,共编码699个氨基酸,理论分子质量为82.76 ku,理论等电位点为5.38,为疏水性蛋白,无信号肽,不属于分泌蛋白,经序列对比发现其与牛和猪的同源性为97%。

In order to study the function of long-chain fatty acyl-Co A 1（ ACSL1） in sheep,the coding sequences（ CDS） and 3＇ untranslated regions were amplified by RACE from total RNA of ovine liver. The CDS of ACSL1 was cloned and analyzed with the bioinformatics software. The results showed that the sequence length of ACSL1 was 2 100 bp encoding one opening reading frame with699 amino acid residues. The molecular weight of ACSL1 was 82. 76 ku,and the theoretical p I was5. 38. ACSL1 was a hydrophobic protein without signal peptide,and did not belong to a secreted protein. Sequence analysis found that the homology between sheep and pig or cattle reached 97%.