糖和植物生长调节剂对万寿菊花色素苷合成的影响

Effects of Sugars and Plant Growth Regulators on Anthocyanin Synthesis in Tagetes erecta

该文探讨了不同浓度糖和植物生长调节剂对万寿菊(Tagetes erecta)花瓣着色的影响。试验以P2期舌状花序为材料,分别在含有不同浓度蔗糖、葡萄糖和生长素、细胞分裂素的固体培养基(MS)上进行培养。第9天取样,利用紫外分光光度计法测定培养花瓣花色素苷积累的含量。结果显示,不同浓度的糖对万寿菊花色素苷的合成都有促进作用,其中,3%葡萄糖和3%蔗糖对花色素苷作用最明显(F_(0.05)<F<F_(0.01));植物生长调节剂对万寿菊花色素苷的合成也有促进作用,但在生长素NAA和细胞分裂素6-BA共同作用下,花色素苷积累高于单一植物生长调节剂的作用,0.2mg·L^(-1)的NAA和1.0mg·L^(-1)的6-BA对花色素苷合成的作用最显著。

Effects of sugars and hormones on Tagetes erecta pigmentation were investigated.Inflorescences or ray floret（rf）at stage P2 were detached and incubated on the medium（MS）under natural light.The ultraviolet spectrophotometer is used to determine the harvesting of anthocyanin glucoside determination in rf petal pigments at the 9th day.The results showed that sugars and hormones exposure induced anthocyanin accumulation.Effects of 3% glucose or 3% sucrose on anthocyanin was the most obvious（F_（0.05）FF_（0.01））.Anthocyanin accumulation was significantly higher with auxin NAA and cytokinin 6-BA interaction,than that of the function of single hormone,and 0.2mg·L-1 of NAA with 0.2mg·L-1 of 6-BA on flower pigment were the most significant synthesis of anthocyanin.