J亚群禽白血病病毒受体基因NHE1的遗传多态性分析

Analysis on genetic polymorphism of avian leukosis virus subgroup J receptor gene NHE1

为发掘J亚群禽白血病病毒（ALV-J）受体基因NHE1潜在的遗传抗性位点,采用PCR技术分段扩增NHE1受体基因序列,并分析其遗传多态性。结果显示,在黄羽肉鸡NHE1基因外显子区域发现了16个SNP突变,分别为c.309G〉C,c.11653C〉T,c.11689G〉A,c.11917C〉T,c.11946C〉T,c.13405C〉A,c.13429T〉C,c.13438T〉C,c.14108A〉C,c.14516A〉G,c.14522G〉A,c.14654C〉T,c.15834G〉A,c.15873G〉A,c.17560C〉Gandc.17770G〉A。进一步分析表明这些SNP突变均为同义c SNP,未引起氨基酸发生改变;在黄羽肉鸡NHE1基因内含子区域发现了SNP和插入/缺失突变,NHE1基因内含子2c.12239~12240和c.13114~13115分别存在AGGGCTGC和TGAAGCC插入突变,内含子11c.17073~17091存在TGTCCTGTGTCCTCCCTGC缺失突变;在ALV-J攻毒后阴性鸡和阳性鸡的NHE1基因外显子区域均发现c.309G〉C、c.11653C〉T、c.13405C〉A、c.13438T〉C、c.14108A〉C和c.14516A〉G6个SNP突变,内含子区域也均发现与黄羽肉鸡相同的插入/缺失突变。研究结果表明,发现的NHE1受体基因遗传变异位点不能作为ALV-J潜在的遗传抗性位点。

To explore the potential genetic resistance loci in avian leukemia virus subgroup J （ALV-J） receptor gene NHE1,the sequence of NHE1 receptor gene was amplified by PCR technique,and the genetic polymorphism of NHE1 gene was analyzed. The results indicated that 16 SNP mutations,including c.309 G〉C,c.11653 C〉T,c.11689 G〉A,c.11917 C〉T,c.11946 C〉T,c.13405 C〉A,c.13429 T〉C,c.13438 T〉C,c.14108 A〉C,c.14516 A〉G,c.14522 G〉A,c.14654 C〉T,c.15834 G〉A,c.15873 G〉A,c.17560 C〉G and c.17770 G〉A,were found within the exon region of NHE1 gene in yellow broilers,these SNPs were synonymous with c SNP and the changes of amino acid were not caused by further analysis. In addition,SNPs and insertion/deletion mutations were also found within the intron region of NHE1 gene in yellow broilers,the AGGGCTGC was inserted between c.12239 and 12240 and the TGAAGCC was inserted between c.13114 and 13115 within the intron 2 of NHE1 gene,respectively,and theTGTCCTGTGTCCTCCCTGC among c.17073-17091 was deleted in intron 11 of NHE1 gene. Furthermore,6 SNPs c.309 G〉C,c.11653 C〉T,c.13405 C〉A,c.13438 T〉C,c.14108 A〉C and c.14516 A〉G within the exon region,as well as insertion/deletion mutations within the intron region that were same with yellow broilers,were found in NHE1 gene of the negative and positive chickens after inoculated by ALV-J. The results demonstrated that the genetic mutation loci found in receptor gene NHE1 may not be used as potential genetic resistance loci of ALV-J.