精神分裂症样小鼠血液中miRNA-132的表达分析

Analysis on blood miRNA-132 expression in schizophrenia mice

目的使用地卓西平马来酸盐(MK-801)腹腔注射制备精神分裂症小鼠模型,研究精神分裂症小鼠外周血中miRNA-132的异常表达情况。方法选取成年雄性昆明小鼠,随机分为注射生理盐水组(对照组)和注射MK-801组(实验组),观察小鼠的行为学改变,用实时荧光定量聚合酶链反应(RT-PCR)法检测各小鼠血液中miRNA-132的表达情况。结果 (1)实验组小鼠刻板行为评分与对照组比较有显著增高(P<0.05),自发活动增加显著,表现为直立数明显减少而走格数明显增多(P<0.05),符合精神分裂症模型小鼠的表现。(2)与对照组相比,实验组miRNA-132表达下调,差异有统计学意义(P<0.05)。结论 miRNA-132在精神分裂症小鼠血液中有异常表达。

Objective To prepare the schizophrenia mouse model by using peritoneal injection of dizocilpine maleate（MK-801）,then to study the peripheral blood miRNA-132 abnormal expression in schizophrenia mouse model.Methods Adult male Kunming mice（KM）were selected and randomly divided into the normal saline group（control group）and MK-801group（experimental group）.The behaviors changes were observed.Real-time PCR method was used to detect the miRNA-132 expression in peripheral blood.Results （1）Compared with the control group,the stereotyped behavior score in the experimental group was increased significantly（P〈0.05）,the spontaneous activity was increased significantly（P0.05）,showing that the up-right number was significantly decreased,while the walking-grid number was significantly increased（P〈0.05）,which was consistent with the manifestations of schizophrenia mouse model.（2）Compared with the control group,the miRNA-132 expressed in the experimental group was down-regulated,the difference was statistically significant（P〈0.05）.Conclusion miRNA-132 is abnormally expressed in blood of schizophrenia mouse.