基于Rho/ROCK信号通路的麦粒灸对自发性高血压大鼠主动脉血管内皮功能保护作用机制探讨

Discussion on the Protection of Aortic Endothelial Function Based on the Rho/ROCK Signaling Pathway of Spontaneous Hypertensive Rats After Grain-sized Moxibustion

目的观察麦粒灸对自发性高血压大鼠(SHR)降压作用及主动脉血管内皮功能的保护并探讨其可能的机制`。方法将SHRs随机分为模型组和麦粒灸组,WKY大鼠设为空白组。模型组、空白组不施灸,麦粒灸组实行麦粒灸治疗。观测各组大鼠血压、血清一氧化氮(NO)含量、主动脉形态学改变。采用Western blot法和qPCR法测定各组大鼠主动脉组织中RhoA、ROCK1和RhoA、ROCK1mRNA表达水平。结果与模型组相比,麦粒灸组血压下降,主动脉内膜增生不明显,仅部分内膜脱落,血清NO含量明显上升,主动脉RhoA、ROCK1和RhoA、ROCK1 mRNA含量显著下降,差异均有统计学意义(P<0.05)。结论麦粒灸能有效降低SHR血压,保护主动脉血管内皮功能,其机制可能与抑制Rho/ROCK信号通路表达相关。

OBJECTIVE To investigate the effect of decreasing blood pressure by moxibustion with seed-sided moxa cone of spontaneously hypertensive rat（SHR）and the protection of vascular endothelial function,and discuss its possible mechanism.METHODS SHRs were randomly divided into a model group and a moxibustion group.WKY rats were regard as bland group.Rats in model group and bland group did not received moxibustion.Rats in the moxibustion group received moxibustion therapy.Observe the blood pressure,the content of nitric oxide（NO）in serum and aortic morphology change of all groups.Samples of aorta were isolated for Western blotting and the qPCR to identify Rho A,ROCK1 and Rho A,ROCK1 mRNA expression levels.RESULTS Compared with model group,moxibustion group had a decreasing of blood pressure,aortic Rho A,ROCK1 and Rho A,ROCK1 mRNA expression,while the NO content in serum increased obviously,the differences were statistically significant（P〈0.05）And the aortic intima hyperplasia was not obvious,just only part of it shed.CONCLUSIONMoxibustion with seed-sided moxa cone can reduce the blood pressure of SHR,protect its vascular endothelia function.The mechanism may be related to the inhibition of the expression of Rho/ROCK signal pathway.