青春期牙龈炎牙龈卟啉单胞菌rag位点基因分型与致病力分析

Detection of Porphyromonas gingivalis rag genotypes in adolescence gingivitis patients

目的:分析不同rag基因型牙龈卟啉单胞菌在青春期牙龈炎中的分布状况。方法:分别选取青春期牙龈炎(GI≥1,PD≥3 mm)64例及牙周健康者(GI=0,PD<3 mm)56例作为研究对象,采用16S r DNA聚合酶链反应(PCR)法检测受试者龈沟液标本中牙龈卟啉单胞菌及致病岛rag基因,分析其与临床指标之间的相互关系。结果:病例组病变位点和对照组牙龈卟啉单胞菌阳性检出率分别为65.63%(42/64)、33.93%(19/56)(P<0.05)。牙龈卟啉单胞菌阳性患者(n=37)的rag基因型检出率依次为rag-3(16/37)、rag-1(9/37))、rag-4(7/37)和rag-2(5/37)。结论:青春期牙龈炎患者龈沟液中牙龈卟啉单胞菌存在rag基因多态性,其中rag-1和rag-3基因型牙龈卟啉单胞菌与青春期牙龈炎的发生发展关系密切。

AIM： To investigate the distribution of Porphyromonas gingivalis（ Pg） rag genotypes in adolescence gingivitis patients. METHODS： 64 adolescent gingivitis patients（ GI ≥1,PD ≥3 mm） and 56 gingivally healthy controls（ GI=0,PD 3mm） were included. Gingival crevicular fluid（ GCF） was collected from all subjects.Pg and rag genotypes of Pg in GCF were detected by PCR method. RESULTS： The detection rate of Pg in GCF of the patients and healthy controls was 65. 63%（42 /64） and 33.93%（19 /56） respectively（ P〈0. 05）. In Pg postive patients（n =37）,the rag genotypes in Pg positive samples was in the following order： rag- 3（ 16 /37）,rag- 1（9 /37）,rag- 4（ 7 /37） and rrag- 2（5 /37）,in the controls（n=10） was rag- 3（ 5 /10）,rag- 2（2 /10）,rag- 4（2 /10） and rag- 1（1 /10）（P〈0.05）. CONCLUSION： Various rag genotypes can be found in GCF of adolescent gingivitis patients. Rag- 1 and rag- 3 might have more close correlation with the development of adolescent gingivitis.