Total soy saponins improve the antioxidant capacity of the myocardium and exercise ability in exhausted rats

大豆总皂苷改善力竭大鼠心肌抗氧化能力与运动能力（英文）

Purpose：The aim of the present study was to investigate the impact of total soy saponins（TS） on the myocardial antioxidant capacity in rats exercised to exhaustion.Methods：The one-time exhausted treadmill model was used.All rats were divided into 4 groups：the control group,the TS group,the exhausted group,and the TS exhausted group.The TS and TS exhausted groups were fed TS at a dosage of 20 mg/kg body weight,once a day,for 2 weeks.The exhausted group was given a placebo,and the control group was not given any treatment.The treadmill speed was set at 30 m/min,and the rats（exhausted and TS exhausted groups） were trained at this speed until exhausted.The rats were decapitated and anatomized immediately after exhausted.A 10% homogenate of the myocardial tissue was prepared.Results：TS significant y increased the exercise time by 20.62%（p〈0.05）.As compared with the control group,the enzyme activities for catalase（CAT）,glutathione peroxidase（GSH-Px）,and glutathione reductase（GR） were significant y enhanced in the TS group（p〈0.01）;GR and GSH-Px activity was significant y enhanced in the TS exhausted group（p〈0.01）;malondialdehyde（MDA） levels were significant y decreased in the TS exhausted group（p〈0.05）.As compared with the exhausted group,the GSH-Px activity was significant y enhanced in the TS exhausted group（p〈0.01）;CAT,GSH-Px,and GR activities were significant y enhanced in the TS group（p〈0.01）.As compared with the TS group,the CAT and GR activity in the TS exhausted group was significant y decreased（p〈0.01）.Conclusion：TS can improve the exercised rats＇ antioxidant activity in their cardiac muscle to varying degrees,decrease MDA and serum AST and LDH levels,increase the exercise time,and delay the occurrence of sports fatigue.

目的:本实验旨在研究大鼠运动至力竭过程中,大豆总皂苷(TS)对心肌抗氧化能力的影响。方法:用跑台一次运动力竭模型,将受试大鼠分为4组:对照组、TS组、力竭组、TS力竭组。按每千克体重20毫克、每天一次的剂量持续两周喂服TS组和TS力竭组大鼠。力竭组喂服安慰剂,对照组不给予任何处理。将跑步机速度预设为30 m/min,让力竭组和TS力竭组大鼠进行跑台运动直至力竭,立即处死并制备10%的心肌组织匀浆。结果:与力竭组相比,TS力竭组运动时间显著延长20.62%(p<0.05)。与对照组相比,TS组CAT、GSH-Px和GR酶活性显著增强(p<0.01),TS力竭组GSH-Px与GR酶活性显著增强(p<0.01)而MDA水平显著降低(p<0.05)。与力竭组相比,TS力竭组GSH-Px活性显著增强(p<0.01),TS组CAT、GSH-Px和GR酶活性均显著提高(p<0.01)。与TS组相比,TS力竭组CAT和GR活性显著增强(p<0.01)。结论:TS可不同程度地改善运动大鼠的心肌抗氧化活性,降低大鼠心肌MDA水平、血清AST和LDH水平,延长运动时间并延缓运动疲劳的发生。