太子参提取物对3T3-L1前脂肪细胞分化的影响

Effects of Pseudostellaria Heterophylla( Miq. ) Pax et Hoffm. Extract on3T3-L1 Pre-adipocytes Differentiation

目的探索太子参提取物对3T3-L1前脂肪细胞分化的影响。方法采用超声提取法获得太子参乙醇提取物,再分别用石油醚、乙酸乙酯、水饱和正丁醇进行连续萃取,用得到的太子参各溶剂萃取层作用于3T3-L1前脂肪细胞分化模型,以罗格列酮为阳性对照药,并通过油红O染色观察其对前脂肪细胞分化过程的影响。结果太子参乙醇提取物及各极性溶剂萃取层在0.4μg·m L-1和0.8μg·m L-1两个浓度下均能在一定程度上促进前脂肪细胞的分化过程,而且乙酸乙酯萃取层活性最强。结论太子参乙酸乙酯萃取层具有较好的促进3T3-L1前脂肪细胞分化的活性。

OBJECTIVE To investigate influence of Pseudostellaria heterophylla（ Miq.） Pax et Hoffm. extract on 3T3-L1 pre-adipocytes differentiation. METHODS Ethanol extract of P. heterophylla（ Miq.） was isolated by ultrasonic extraction method,and then was extracted respectively by petroleum ether,ethyl acetate,water saturated nbutanol and water. The obtained ethanol extract and its solvent extraction layers were applied in 3T3-L1 pre-adipocyte differentiation model and stained by oil red O,taking rosiglitazone as positive control drug. RESULTS Ethanol extract and its solvent extraction layers of P. heterophylla（ Miq.） under the concentration of 0. 4μg·m L- 1and 0. 8μg·m L- 1promoted pre-adipocyte differentiation in a certain extent,moreover,ethyl acetate extract exhibited strongest activity. CONCLUSION Ethyl acetate extract of P. heterophylla（ Miq.） possesses good property which can promote differentiation of 3T3-L1 preadipocytes.