细菌潜生体与盆腔脓肿发病相关性研究

Study on the association between pelvic abscess and the cryptic growth cell

目的探讨大肠埃希菌潜生体(CGC)的形成机制及其与盆腔脓肿发病的相关性。方法构建盆腔炎性疾病(PID)模型:以雌性SD大鼠为研究对象。开腹在肠系膜处接种大肠埃希菌,观察生殖系统的病理变化,确定PID的急性加重期。构建PID急性加重期模型:分4组,对照组1组,抗生素组3组。注射1/2浓度抗生素,模拟临床非规范化应用。切片观察病灶处病理改变,镜下观察CGC的产生。结果 SD大鼠PID模型病灶处组织病理损伤程度与时间呈正相关。模型中炎症的病理转归分为急性期与慢性期。急性加重期界定为建模后7~17 d,但均无化脓现象。构建PID急性加重期内动物模型,使用1/2浓度头孢菌素的模型组病灶出现化脓反应,组织损伤严重。脓液中检测出革兰阴性、直径3~15μm的纤细大肠埃希菌CGC。结论不规范的抗生素治疗PID可诱导CGC形成,有诱发盆腔脓肿的可能。

Objective To study the antibiotic function of inducing the E.coli to Cryptic Growth Cells（CGC）and its correlation with pelvic abscess.Methods Establish the models of pelvic inflammatory disease（PID）;Use the SD female rats as research subjects;inculate the E.coli on the mesosalpinx;observe the pathological changes of reproductive system;determine the acute exacerbation.Establish PID acute exacerbation models and divide them into 4 groups：one for control and the other 3 for experiment.Use 1/2 concentration antibiotics to induce the CGC to simulate denormalized treatment.Observe the pathological change and CGC. Results In the PID models,the pathological change and time had positive correlation.Among these models,the course of PID was divided into acute and chronic stage.The acute exacerbation was defined as the range between 7th to17 th days after modeling,but there was no suppuration during that time.CGC was found from the models which were injected 1/2 concentration of cephalosporins during the acute exacerbation.At the same time,lesions appeared suppuration and serious tissue damage.Conclusion CGC can be induced by nonstandard antibiotic treatment for PID,which may induce pelvic abscess.