高效液相色谱法同时测定化妆品中的10种美白活性成分及2种禁用成分

Simultaneous Determination of 10 Whitening and 2 Prohibited Components in Cosmetics by High Performance Liquid Chromatography

建立了同时测定化妆品中10种美白活性成分及2种禁用成分的高效液相色谱分析方法。水基、乳液等含油脂较少的样品采用0.02 mol/L磷酸二氢钾溶液(pH 6.0)直接提取;油脂含量高的样品及蜡基、粉基类的样品先加入2.5 m L二氯甲烷溶解后再用0.02 mol/L磷酸二氢钾溶液(pH 6.0)提取。提取液在9 500r/min下离心后用0.22μm滤膜过滤。样品采用Eclipse XDB-C_(18)色谱柱为固定相,以0.02 mol/L磷酸二氢钾溶液(pH 6.0)和甲醇溶液为流动相,梯度洗脱,流速为1.0 m L/min,柱温为25℃,使用二极管阵列检测器(DAD)进行检测,检测波长为230 nm和250 nm,外标法定量。结果显示:12种化合物在2.5~100 mg/L范围内线性关系良好,相关系数(r)均大于0.999 0。方法的定量下限(以信噪比为10计)为0.006 5%~0.025%,添加水平为0.025%~0.5%时回收率为87%~102%,相对标准偏差均小于4%。该方法前处理简单、回收率高、精密度好,适用于化妆品中10种美白活性成分及2种禁用成分的快速测定。

A method was established for the detection of 10 whitening and 2 prohibited components in cosmetics by high performance liquid chromatography（HPLC）.Low fat cosmetics samples such as make up water and lotion were extracted directly with 0.02 mol/L potassium dihydrogen phosphate solution（pH 6.0）.High fat cosmetics samples and wax based and powder cosmetics were well dispersed with 2.5 mL dichloromethane first,then extracted with 0.02 mol/L potassium dihydrogen phosphate solution（pH 6.0）.The sample solution were centrifuged at a speed of 9 500 r/min,then filtered through a 0.22 μm syringe filter.The filtrate were analyzed on an Eclipse XDB-C18 column （250 mm×4.6 mm,i.d 5 μm） using 0.02 mol/L potassium dihydrogen phosphate （pH 6.0）-methanol as mobile phase at a flow rate of 1.0 mL/min.The temperature of the column was set at 25 ℃.The detection wavelengths were 230 nm and 250 nm.The standard working curves of 12 compounds had good linear relationships（r〉0.999 0） in concentration range of 2.5-100 mg/L.The quantitation limits（S/N=10） of 12 compounds were in the range of 0.006 5%-0.025%.The recoveries of 12 analytes at spiked levels of 0.025%-0.5% were in the range of 87%-102%with relative standard deviations less than 4%.With the advantages of simplicity,high recovery and good precision, this methed was suitable for the determination of 10 whitening and 2 prohibited components in cosmetics.