Gadd45α基因敲减对缺氧/复氧下人绒毛外滋养细胞迁移侵袭能力的影响

Effects of Gadd45α-knockdown on human extravillous trophoblast cell migration and invasion functions under hypoxia/re-oxygenation

目的·探讨沉默生长阻滞和DNA损伤45α(Gadd45α)基因对缺氧/复氧下人绒毛外滋养细胞迁移侵袭能力的影响。方法·用靶向Gadd45α基因的sh RNA慢病毒感染人绒毛外滋养细胞HTR8/SVneo,敲减Gadd45α的基因表达;采用氧化应激模型体外模拟子痫前期,观察细胞生物学功能的改变。实验分成4组:对照组、缺氧/复氧组、Gadd45α敲减+缺氧/复氧组和慢病毒阴性对照+缺氧/复氧组。采用早孕绒毛外植体实验观察Gadd45α基因敲减对氧化应激下人绒毛外滋养细胞生物学功能的影响,Western blotting法检测各组细胞Gadd45α蛋白水平变化,Transwell实验观察细胞侵袭和迁移能力,明胶酶谱法检测细胞培养上清液中基质金属蛋白酶2/9的活性。结果·缺氧/复氧可使HTR8/SVneo细胞中Gadd45α表达增加,进而细胞的迁移和侵袭能力下降;而Gadd45α干扰可提高基质金属蛋白酶2/9的活性进而提高氧化应激下绒毛外滋养细胞的迁移和侵袭能力。结论·Gadd45α基因敲减对氧化应激下人绒毛外滋养细胞迁移侵袭能力具有促进作用。

Objective · To investigate the effects of growth arrest and DNA damage 45 alpha （Gadd45α） on the migration and invasion function of human extravillous trophoblast cells under hypoxia/re-oxygenation （H/R）. Methods · Human extravillous trophoblast cells were infected by shRNA lentivirus targeting Gadd45α gene, to knock down Gadd45α gene expression. Then the oxidative stress model of preeclampsia was used in vitro to observe the changes of cell biological functions. The experiments were divided into 4 groups, nontreated group, hypoxia/re-oxygenation group, shRNA Gadd45α＋H/R group and shRNA negative control＋H/R group. Human villous explant experiments were used to determine the effects of silencing Gadd45α on human extravillous trophoblast cell under oxidative stress. Protein expression of Gadd45α was identified by Western blotting. Changes of cell migration and invasion were detected by transwell migration and Matrigel invasion assay. Gelatin zymography was used to detect the expression of matrix metalloproteinase （MMP） -2/9 in culture medium. Results · Hypoxia/re-oxygenation can increase the expression of Gadd45α in HTR8/SVneo cells and damage the trophoblast cell migration and invasion. Knocking down Gadd45α can increase the activities of MMP2/9, which can increase the cell migration and invasion. Conclusion · Knockdown of Gadd45α gene has promoted cell migration and invasion function of human extravillous trophobalst cells under oxidative stress.