贝类腹泻性毒素现场高通量前处理及快速检测仪器设计

Design of In-Suit High Throughput Pretreatment and Detection System for Diarrhetic Shellfish Poisons

为实现贝类腹泻性毒素的现场快速检测,本论文设计了一种现场高通量前处理装置及基于移动终端的快速检测系统。通过使用酶联免疫反应技术对腹泻性贝毒进行显色反应,结合基于移动终端的快速检测装置,采集显色反应结果图像,实现准确快速的检测结果分析。采用所设计的前处理装置与实验室手工前处理标准方法对比,其回收率分别为89%和93%,结果表明,本文设计的前处理装置能够满足现场检测分析的需求。通过加标样品检测,采用移动终端的快速检测系统测量标准差为0.13,平均回收率89.5%。实验结果表明,该检测系统的准确性和重复性满足实际检测的需求。最后,针对实际样品,与酶标仪检测结果相比较,表明了所设计的现场高通量前处理装置及快速检测系统能达到对贝类腹泻性毒素现场快速精确检测,为贝类腹泻性毒素现场检测提供了新的方法和仪器。

In order to achieve the requirement of diarrhetic shellfish poison（DSP）in-suit detection,we design a high throughput pretreatment system and a mobile terminal based detection system. Our system can make fast and accurate DSP detection by analyzing image of chromogenic reaction come from ELISA method. The rate of recovery by our pretreatment system and by hand are 89% and 93% respectively,it means that our pretreatment system can meet the follow-up detection requirement. Detect the prevented samples by our detection system, the standard deviation is approximately 0.13 and average rate of recovery is 89.5%,it can satisfy the accuracy and repeatability of detection. In the detection of actual samples,comparing our results with the results come from microplate reader,it shows that our pretreatment and detection system can do a fast and accurate in-suit work and provide a new method for the detection.