扶正解毒通络方对人肝癌HepG2细胞MMP-2、MMP-9表达及细胞侵袭作用的影响

Effect of Fuzheng Jiedu Tongluofang on MMP-2 and MMP-9 and invasion of human hepatoma HepG2 cells

目的:探讨扶正解毒通络方对肝癌HepG2细胞侵袭作用和基质金属蛋白酶(Matrix metallo proteinases,MMP)-2、MMP-9表达水平的影响及可能的作用机制。方法:CCK8实验检测扶正解毒通络方对HepG2细胞活性影响;Transwell侵袭实验检测扶正解毒通络方对HepG2侵袭能力的影响;ELISA法检测扶正解毒通络方干预HepG2细胞后MMP-2和MMP-9的表达水平。结果:CCK8实验结果显示,扶正解毒通络方对HepG2细胞意志呈剂量时间相关性。Transwell侵袭实验显示,2.65 mg/ml扶正解毒通络方对HepG2细胞侵袭力抑制率为52.45%(P<0.05)。ELISA检测结果显示,扶正解毒通络方干预后HepG2细胞上清液中MMP-2和MMP-9表达水平下降(P<0.05)。结论:扶正解毒通络方可以抑制肝癌HepG2细胞的生长和侵袭,其机制可能是通过下调MMP-2、MMP-9在肝癌细胞HepG2的表达。

Objective： To investigate the effect and mechanism of Fuzheng Jiedu Tongluofang on the expression of MMP-2,MMP-9 and invasion of human hepatoma HepG2 cell. Methods： Hepatioma HepG2 cell was treated with Fuzheng Jiedu Tongluofang.The cell viability was measured by CCK8. The invasion of HepG2 was detected by Transwell assay. The expression of MMP-2 and MMP-9 was analyzed by ELISA. Results： In the CCK8 assay,Fuzheng Jiedu Tongluofang could inhibit the proliferation of HepG2 cells at a density and time measure. In the Transwell assay,the inhibitory rate of invasion was 52. 45% when treated with Fuzheng Jiedu Tongluofang at a concentration of 2. 65 mg/ml. The ELISA assay indicated that the expression of MMP-2 and MMP-9 decreased significantly after treated with Fuzheng Jiedu Tongluofang（ P0. 05）. Conclusion： Fuzheng Jiedu Tongluofang suppressed the invasion of HepG2 with the possible mechanism of down-regulating the expression of MMP-2 and MMP-9 in HepG2.