HBV相关肝细胞癌肿瘤组织中热休克蛋白mRNA表达水平与肿瘤分期的相关性分析

Correlation between mRNA expression of heat shock protein in tumor tissue and tumor stage in patients with HBV-related hepatocellular carcinoma

目的分析肝细胞癌(HCC)肿瘤组织中热休克蛋白(HSP)mRNA表达水平与HCC肿瘤分期的相关性。方法从NCBI网站的基因表达数据库(GEO)下载GSE14520数据集。从GSE14520基因数据库中获取HSP基因25种,通过与数据库下载的临床资料比对,纳入具有HSP基因mRNA表达水平和有对应临床资料的220例HCC患者。提取220例患者癌组织HSP基因的mRNA表达水平及对应的TNM分期及BCLC分期并进行相关性分析。采用Kruskal-Wallis H非参数检验进行多组间比较,采用单因素及多因素logistic回归分析HSP与HCC肿瘤分期的相关性。结果 HSPA12A、HSPA5、HSP90AA1与HSPA13 mRNA随着TNM分期增高,表达逐渐升高(P值均<0.05);HSP90AA1 mRNA随着BCLC分期增高,表达逐渐升高(P<0.05)。HSPA4L(OR=1.019,P=0.005)、HSPA12A(OR=1.077,P=0.026)与HSP90AA1(OR=1.001,P=0.002)是影响TNM分期的危险因素;HSPA9 mRNA升高与HCC患者TNM分期呈负相关(OR=0.995,P=0.011)。HSPH1(OR=1.005,P=0.041)、HSPA1B(OR=1.002,P=0.048)与HSPA5(OR=1.000,P=0.001)是影响BCLC分期的危险因素;HSPA9 mRNA升高与肝癌BCLC分期呈负相关(OR=0.995,P=0.005)。结论 HSP与HCC患者肿瘤临床分期存在一定相关性。

Objective To investigate the correlation between mRNA expression of heat shock protein （HSP） and tumor stage in patients with HBV -related hepatoeellular carcinoma （HCC）. Methods The GSE14520 data set was downloaded from gene expression omnibus in the website of the National Center of Biotechnology Information. A total of 25 HSP genes were obtained from the GSE14520 data, and after the comparison with the clinical data downloaded from the database, 220 HCC patients with mRNA expression of HSP and corresponding clinical data were enrolled. The mRNA expression of HSP in tumor tissues was measured, the TNM stage and Barcelona Clinic Liver Cancer （BCLC） stage were determined for each patient, and a correlation analysis was performed. The Kruskal - Wallis H non - parametric test was used for comparison between multiple groups, and univariate and multivariate logistic regression analyses were used to investigate the correla- tion between HSP and HCC tumor stage. Results With the increase in TNM stage, the expression of HSPA12A, HSPA5, HsPg0AA1, and HSPA13 gradually increased （all P 〈0.05 ）, while the expression of HSP90AA1 gradually increased with the increase in BCLC stage （P 〈 0. 05 ）. HSPA4L （ OR = 1. 019, P = 0. 005 ）, HSPA12A （ OR = 1. 077, P = 0.026）, and HSP90AA1 （ OR = 1. 001, P = 0.002） were risk fac- tors for TNM stage. HSPA9 was negatively correlated with TNM stage （ OR = 0.995, P = 0.011 ）. HSPH1 （ OR = 1. 005, P = 0. 041 ）, HS- PAl B （ OR = 1. 002, P = 0. 048 ）, and HSPA5 （ OR = 1. 000, P = 0.001 ） were risk factors for BCLC stage, and HSPA9 was negatively corre- lated with BCLC stage （ OR = 0.995, P = 0.005 ）. Conclusion There is a certain correlation between HSPs and tumor stage in HCC patients.