侵袭性牙周炎龈沟液中牙龈卟啉单胞菌与齿垢密螺旋体对有机酸影响研究

Influence of Porphyromonas gingivalis and Treponema denticola in gingival crevicular fluid of patients with aggressive periodontitis on organic acid

目的研究侵袭性牙周炎(A_gP)患者龈沟液中牙龈卟啉单胞菌(Porphyromonas gingivalis,P_g)与齿垢密螺旋体(Treponema denticola,Td)对有机酸的影响。方法对2012年3月至2015年2月河南大学第一附属医院诊治的40例侵袭性牙周炎患者(A_gP组)和体检结果为牙周健康的40名志愿者(健康对照组)进行研究。选择受检者口腔同一象限中一颗牙齿的颊侧中心点作为取样点,收集龈沟液,记录菌斑指数(PLI)、探诊深度(PD)、龈沟出血指数(SBI)和附着丧失(AL),采用毛细管电泳仪(CE)检测样本有机酸浓度。P_g和Td检测方法为聚合酶链式反应(PCR)。对检测出阳性的扩增目的条带灰度值进行定量分析,采用扩增条目的条带和DNA分子量标准中最接近条带的单位面积内的灰度比值表示目的基因的相对量,将其定为PCR产物量。结果 A_gP组PLI、PD、AL、有机酸浓度、P_g/Td检出率、P_g/Td产物量等均高于健康对照组(P<0.05);A_gP组P_g阳性检出点丁酸浓度明显高于阴性检出点(P<0.05),Td阳性检出点有机酸浓度均高于阴性检出点(P<0.05)。结论 A_gP患者龈沟液内有机酸浓度在一定水平上体现了P_g和Td的产物量,在A_gP的生成和发展中具有一定作用。

 Objective To investigate the influence of Porphyromonas gingivalis （Pg） and Treponema denticola （Td） in gingival crevicular fluid （GCF） of patients with aggressive periodantitis （AgP） on organic acid.Methods Forty cases of AgP and 40 healthy volunteers in our hospital were selected and GCF was collected.GCF in booth of same quadrant of patients was collected,and plaque index （PLI）,sampling location,probing depth （PD）,sulcus beeding index （SBI） and attachment loss （AL） were recorded.CE was used to detect the concentration of organic acid;PCR was used to detect Pg and Td.The gray value of amplification entry stripe,which was detected positive,was given quantitative analysis;amplification target bars and brightness ratio showed the relative volune of target gene,which was PCR product quantity.Resuits PLI,PD,AL,concentration of acid,Pg/Td detection rate and Pg/Td product quantity of AgP group were higher than those of healthy group （P 〈 0.05）;Pg butyric acid concentration at positive detection point of AgP group was higher than that at negative detection point （P 〈 0.05）;organic acid concentration at Td positive detection point was higher than that at negative group（P 〈 0.05）.Conclusion Organic acid concentration in GCF of AgP patients shows product quantity of Pg and Td,which plays a certain guiding role in organic acid concentration of AgP.