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人参皂甙Rg3对人乳腺癌MDA-MB-231细胞生长的抑制作用及其机制 被引量:3

Inhibition effects and its mechanisms of ginsenoside Rg3 on the proliferation of human breast cancer cell line MDA-MB-231
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摘要 [目的]研究人参皂甙Rg3对人乳腺癌MDA-MB-231细胞生长的抑制作用及其机制.[方法]采用MTT比色法检测人参皂甙Rg3对人乳腺癌MDA-MB-231细胞生长的抑制作用.利用流式细胞仪检测细胞凋亡及周期变化.建立人乳腺癌MDA-MB-231细胞裸鼠移植瘤模型,观察不同浓度的人参皂甙Rg3对裸鼠的体质量及肿瘤增殖抑制率的影响.采用Western blot方法检测肿瘤组织中procaspase-3和procaspase-9蛋白表达水平.[结果]人参皂甙Rg3可明显抑制人乳腺癌MDA-MB-231细胞的生长(P<0.05),且抑制作用呈剂量和时间依赖性;明显促进MDA-MB-231细胞凋亡(P<0.05),可将细胞周期阻滞于G0/G1期.人参皂甙Rg3对人乳腺癌细胞体内的增长具有明显的抑制作用,与对照组比较,人参皂甙Rg3各浓度组proCaspase-3和proCaspase-9蛋白表达水平均明显降低(P<0.05).[结论]人参皂甙Rg3在体内和体外均可抑制人乳腺癌MDA-MB-231细胞生长,机制认为可能与诱导细胞凋亡、改变细胞周期和Caspase-3,Caspase-9活化有关系. OBJECTIVE To study the inhibition effects of ginsenoside Rg3 on the proliferation of human breast cancer cell line MDA-MB-231 and its mechanism.METHODS MTT colorimetric assay was used to detect the inhibition effects.Flow cytometry was used to detect the apoptosis and the change of cell cycle.Nude mouse transplanted tumor model of human breast cancer cell line MDA-MB-231 was set up.The effects of different concentrations of ginsenoside Rg3 on the body mass and tumor proliferation inhibition rate of nude mice were observed.Western blot was used to detect the expressions of proCaspase-3and proCaspase-9in tumor xenografts.RESULTS Ginsenoside Rg3 could significantly inhibit the growth of human breast cancer cell line MDA-MB-231(P 0.05),and the inhibitory effects were dose and time dependent.Ginsenoside Rg3 could obviously promote the cell apoptosis(P0.05),and arrest the cell cycle in G_0/G_1 phase.Ginsenoside Rg3 could significantly inhibit the growth of human breast cancer cell in vivo.As compared with the control group,the expressions of proCaspase-3and proCaspase-9in different dosages of ginsenoside Rg3 groups obviously reduced(P 0.05).CONCLUSION Ginsenoside Rg3 could inhibit the growth of human breast cancer cell line MDA-MB-231 in vitro and in vivo,and the mechanism might be related with the induction of cell apoptosis,the change of cell cycle and the activation of Caspase-3and Caspase-9.
出处 《延边大学医学学报》 CAS 2017年第1期20-23,共4页 Journal of Medical Science Yanbian University
基金 吉林省教育厅资助项目 吉教科合字2015第19号
关键词 乳腺癌 人参皂甙RG3 凋亡 CASPASE-3 CASPASE-9 breast cancer gensenoside Rg3 apoptosis Caspase-3 Caspase-9
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