安徽部分地区规模化猪场伪狂犬病血清流行病学调查

Serological Investigation of Porcine Pseudorabies in Part of Scale Swine Farms in Anhui Province

为了解安徽地区不同规模猪场猪伪狂犬野毒(PRV-gE)的感染情况,为防控猪伪狂犬病(PR)提供科学依据,本调查应用ELISA方法,对2012年8月~2015年12月来自224个不同规模猪场的11 855份血清样品进行PRV-gE抗体检测。结果显示,血清样品总阳性率为14.03%,2012年8月~2015年12月分别为2.76%、5.12%、11.61%、26.61%;育肥猪、哺乳仔猪、母猪、保育猪、后备母猪及公猪依次为31.02%、29.27%、27.46%、22.29%、22.22%、18.52%,皖北、皖南和江淮地区分别为28.59%、9.59%、6.49%。猪场总阳性率为41.96%,大型规模场、中等规模场、小型规模场及散养户分别为4.74%、17.00%、23.15%,皖北、皖南和江淮地区分别为48.48%、35.71%、38.53%。结果表明,安徽地区猪群中PRV感染逐年上升,皖北地区样品阳性率显著高于其他地区,其中小型规模场及散养户的感染尤为严重,育肥猪和种猪带毒是伪狂犬病持续存在的重要原因,养殖方式、疫苗免疫、生物安全等因素均对PR的流行产生影响。

To understand and grasp the wild pseudorabies virus （PRV-gE） infection in different scale breed- ing pig farms in Anhui province, to provide scientific evidences for making effective measures to prevent and control of porcine pseudorabies, this survey used ELISA method, 11855 serum samples from 224 different scale pig farms were detected for specific antibody against pseudorabies virus（PRV-gE） from August 2012 to December 2015 .The result of the survey demonstrated that the total positive rate of serum samples was 14.03% ,from August 2012 to December 2015,the positive rates of the serum samples were 2.76%, 5.12%,11.61% and 26.61%.The positive rates of the serum samples in fattening pigs, suckling piglets, sows,nursery pigs,replacement pigs and boars were 31.02%, 29.27%, 27.46%, 22.29%, 22.22% and 18. 52%,respectively. The positive rates of serum samples in Southern Anhui, Changjiang-huaihe basin and Northern Anhui were 28.59% ,9.59% and 6.49%.The total positive rate of the farms was 41.96% ,the positive rates in large farms, medium farms, small farms were 4.74 %, 17.00 % and 23.15 %, and in Southern Anhui,Changjiang-huaihe basin and Northern Anhui were 48.48 %, 35.71% and 38.53 %. The result revealed that the infection rate of PRV increased year by year in Anhui, the positive rate of samples in Northern Anhui was significantly higher than that in other regions,the infection in small farms was particularly serious,the fattening pigs and boars carrying virus are important reasons for the persistence of pseudorahies.Breeding methods, vaccine immunization, biosecurity and other factors all have impacts on the prevalence of PR.